An extremely promiscuous terpenoid synthase from the Lamiaceae plant Colquhounia coccinea var. mollis catalyzes the formation of sester-/di-/sesqui-/mono-terpenoids

doi:10.1016/j.xplc.2021.100233

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	An extremely promiscuous terpenoid synthase from the Lamiaceae plant Colquhounia coccinea var. mollis catalyzes the formation of sester-/di-/sesqui-/mono-terpenoids
	Li,De-Sen ; Hua,Juan ; Luo,Shi-Hong ; Liu,Yan-Chun ; Chen,Yue-Gui ; Ling,Yi ; Guo,Kai ; Liu,Yan ; Li,Sheng-Hong
	2021
发表期刊	PLANT COMMUNICATIONS
ISSN	2590-3462
卷号	2 期号:5 页码:100233
摘要	Terpenoids are the largest class of natural products with complex structures and extensive bioactivities; their scaffolds are generated by diverse terpenoid synthases (TPSs) from a limited number of isoprenoid diphosphate precursors. Promiscuous TPSs play important roles in the evolution of terpenoid chemodiversity, but they remain largely unappreciated. Here, an extremely promiscuous terpenoid synthase (CcTPS1) of the TPS-b subfamily was cloned and functionally characterized from a leaf-specific transcriptome of the Lamiaceae plant Colquhounia coccinea var. mollis. CcTPS1 is the first sester-/di-/sesqui-/mono-TPS identified from the plant kingdom, accepting C-25/C-20/C-15/C-10 diphosphate substrates to generate a panel of sester-/di-/sesqui-/mono-terpenoids. Engineered Escherichia coli expressing CcTPS1 produced three previously unreported terpenoids (two sesterterpenoids and a diterpenoid) with rare cyclohexanecontaining skeletons, along with four sesquiterpenoids and one monoterpenoid. Their structures were elucidated by extensive nuclear magnetic resonance spectroscopy. Nicotiana benthamiana transiently expressing CcTPS1 also produced the diterpenoid and sesquiterpenoids, demonstrating the enzyme's promiscuity in planta. Its highly leaf-specific expression pattern combined with detectable terpenoid products in leaves of C. coccinea var. mollis and N. benthamiana expressing CcTPS1 suggested that CcTPS1 was mainly responsible for diterpenoid and sesquiterpenoid biosynthesis in plants. CcTPS1 expression and the terpenoid products could be induced by methyl jasmonate, suggesting their possible role in plant-environment interaction. CcTPS1 was localized to the cytosol and may differ from mono-TPSs in subcellular compartmentalization and substrate tolerance. These findings will greatly aid our understanding of plant TPS evolution and terpenoid chemodiversity; they also highlight the enormous potential of transcriptome mining and heterologous expression for the exploration of unique enzymes and natural products hidden in plants.
关键词	terpenoid diversity terpenoid synthase promiscuous enzyme sester-/di-/sesqui-/mono-terpenoids Colquhounia coccinea var mollis GERANYLFARNESYL DIPHOSPHATE SYNTHASE GLANDULAR TRICHOMES DITERPENE SYNTHASE LEUCOSCEPTRUM-CANUM IDENTIFICATION BRASSICACEAE BIOLOGY FAMILY HARBOR GENES
DOI	10.1016/j.xplc.2021.100233
WOS记录号	WOS:000696699600010
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73448
专题	中国科学院昆明植物研究所
作者单位	1.[Li, De-Sen 2.Hua, Juan 3.Luo, Shi-Hong 4.Liu, Yan-Chun 5.Chen, Yue-Gui 6.Ling, Yi 7.Liu, Yan 8.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 9.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China 10.[Guo, Kai 11.Chengdu Univ Tradit Chinese Med, Inst Chinese Med & Pharm, State Key Lab SouthWestern Chinese Med Resources, Chengdu 611137, Peoples R China 12.[Hua, Juan 13.Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110866, Peoples R China 14.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li,De-Sen,Hua,Juan,Luo,Shi-Hong,et al. An extremely promiscuous terpenoid synthase from the Lamiaceae plant Colquhounia coccinea var. mollis catalyzes the formation of sester-/di-/sesqui-/mono-terpenoids[J]. PLANT COMMUNICATIONS,2021,2(5):100233.
APA	Li,De-Sen.,Hua,Juan.,Luo,Shi-Hong.,Liu,Yan-Chun.,Chen,Yue-Gui.,...&Li,Sheng-Hong.(2021).An extremely promiscuous terpenoid synthase from the Lamiaceae plant Colquhounia coccinea var. mollis catalyzes the formation of sester-/di-/sesqui-/mono-terpenoids.PLANT COMMUNICATIONS,2(5),100233.
MLA	Li,De-Sen,et al."An extremely promiscuous terpenoid synthase from the Lamiaceae plant Colquhounia coccinea var. mollis catalyzes the formation of sester-/di-/sesqui-/mono-terpenoids".PLANT COMMUNICATIONS 2.5(2021):100233.

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