Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization

doi:10.1016/j.carbpol.2021.118368

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	Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization
	Chen,Ru ; Yin,Ronghua ; Li,Shanni ; Pan,Ying ; Mao,Hui ; Cai,Ying ; Lin,Lisha ; Wang,Weili ; Zhang,Taocui ; Zhou,Lutan ; Gao,Na ; Luo,Xiaodong; Zhao,Jinhua
	2021
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	270 页码:118368
摘要	Free radical depolymerization is a common method in structural analysis of polysaccharides, the major challenge is the analysis of the cleavage site and characterization of newly formed ends in this reaction. Here, a fucosylated glycosaminoglycan from H. fuscopunctata (HfFG) was depolymerized by H2O2 and a series of oligosaccharides were purified and their structures were elucidated. For non-reducing ends of the trisaccharides were intact GalNAc(4S6S), the cleavage site should mainly be the beta(1,3) linkages between GlcA and GalNAc in the backbone of FG. Meanwhile, the reducing ends of the disaccharides and trisaccharides were almost dicarboxylic acid derivatives of GlcA, possibly arising from oxidative breaking of the C-C bond of GlcA at the reducing ends. In addition, glycosidic linkages in D-GalNAc-beta(1,4)-D-GlcA and L-FucS-alpha(1,3)-D-GlcA located at the reducing end could be cleaved, and the released GalNAc(4S6S) were oxidized to N-acetylgalactosaminic acid.
关键词	Fucosylated glycosaminoglycan Free radical depolymerization Chemical structure Oligosaccharide NMR INTRINSIC TENASE COMPLEX ANTICOAGULANT ACTIVITIES SEA-CUCUMBER CHONDROITIN SULFATE PHYSICOCHEMICAL CHARACTERISTICS FACTOR XASE POLYSACCHARIDES DERIVATIVES HEPARIN
DOI	10.1016/j.carbpol.2021.118368
WOS记录号	WOS:000685075400008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73419
专题	中国科学院昆明植物研究所
作者单位	1.Yunnan Univ, Sch Chem Sci & Technol, Key Lab Med Chem Nat Resource, Minist Educ & Yunnan Prov, Kunming 650091, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Yunnan Inst Tradit Chinese Med & Mat Med, Kunming 650223, Yunnan, Peoples R China 6.Guangxi Inst Tradit Med & Pharmaceut Sci, Nanning 530022, Peoples R China
推荐引用方式 GB/T 7714	Chen,Ru,Yin,Ronghua,Li,Shanni,et al. Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization[J]. CARBOHYDRATE POLYMERS,2021,270:118368.
APA	Chen,Ru.,Yin,Ronghua.,Li,Shanni.,Pan,Ying.,Mao,Hui.,...&Zhao,Jinhua.(2021).Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization.CARBOHYDRATE POLYMERS,270,118368.
MLA	Chen,Ru,et al."Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization".CARBOHYDRATE POLYMERS 270(2021):118368.

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