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| Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization | |
Chen,Ru; Yin,Ronghua; Li,Shanni; Pan,Ying; Mao,Hui; Cai,Ying; Lin,Lisha; Wang,Weili; Zhang,Taocui; Zhou,Lutan; Gao,Na; Luo,Xiaodong ; Zhao,Jinhua
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| 2021 | |
| Source Publication | CARBOHYDRATE POLYMERS
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| ISSN | 0144-8617 |
| Volume | 270Pages:118368 |
| Abstract | Free radical depolymerization is a common method in structural analysis of polysaccharides, the major challenge is the analysis of the cleavage site and characterization of newly formed ends in this reaction. Here, a fucosylated glycosaminoglycan from H. fuscopunctata (HfFG) was depolymerized by H2O2 and a series of oligosaccharides were purified and their structures were elucidated. For non-reducing ends of the trisaccharides were intact GalNAc(4S6S), the cleavage site should mainly be the beta(1,3) linkages between GlcA and GalNAc in the backbone of FG. Meanwhile, the reducing ends of the disaccharides and trisaccharides were almost dicarboxylic acid derivatives of GlcA, possibly arising from oxidative breaking of the C-C bond of GlcA at the reducing ends. In addition, glycosidic linkages in D-GalNAc-beta(1,4)-D-GlcA and L-FucS-alpha(1,3)-D-GlcA located at the reducing end could be cleaved, and the released GalNAc(4S6S) were oxidized to N-acetylgalactosaminic acid. |
| Keyword | Fucosylated glycosaminoglycan Free radical depolymerization Chemical structure Oligosaccharide NMR INTRINSIC TENASE COMPLEX ANTICOAGULANT ACTIVITIES SEA-CUCUMBER CHONDROITIN SULFATE PHYSICOCHEMICAL CHARACTERISTICS FACTOR XASE POLYSACCHARIDES DERIVATIVES HEPARIN |
| DOI | 10.1016/j.carbpol.2021.118368 |
| WOS ID | WOS:000685075400008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/73419 |
| Collection | 中国科学院昆明植物研究所 |
| Affiliation | 1.Yunnan Univ, Sch Chem Sci & Technol, Key Lab Med Chem Nat Resource, Minist Educ & Yunnan Prov, Kunming 650091, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Yunnan Inst Tradit Chinese Med & Mat Med, Kunming 650223, Yunnan, Peoples R China 6.Guangxi Inst Tradit Med & Pharmaceut Sci, Nanning 530022, Peoples R China |
| Recommended Citation GB/T 7714 | Chen,Ru,Yin,Ronghua,Li,Shanni,et al. Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization[J]. CARBOHYDRATE POLYMERS,2021,270:118368. |
| APA | Chen,Ru.,Yin,Ronghua.,Li,Shanni.,Pan,Ying.,Mao,Hui.,...&Zhao,Jinhua.(2021).Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization.CARBOHYDRATE POLYMERS,270,118368. |
| MLA | Chen,Ru,et al."Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization".CARBOHYDRATE POLYMERS 270(2021):118368. |
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| 170-J03-217.pdf（3356KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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