Chemical and biological comparison of different parts of Paeonia suffruticosa (Mudan) based on LCMS-IT-TOF and multi-evaluation in vitro

doi:10.1016/j.indcrop.2019.112028

	Chemical and biological comparison of different parts of Paeonia suffruticosa (Mudan) based on LCMS-IT-TOF and multi-evaluation in vitro
	Pan, Yang 1,2; Gao, Zhen 1,2; Huang, Xiao-Yan 1; Chen, Ji-Jun1,2 ; Geng, Chang-An1
通讯作者	Geng, Chang-An(gengchangan@mail.kib.ac.cn)
	2020-02-01
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
卷号	144 页码:9
摘要	Paeonia suffruticosa Andr. (Mudan) as a famous medicinal and ornamental plant is widely cultivated in China. In the traditional Chinese medicine (TCM) system, only the root bark (Moutan Cortex) is used in the production of crude drugs. The present study aimed to systematically compare the chemical profiles and biological activities of different parts (root bark, root core, old stems, young stems, and leaves) of P. suffruticosa by LCMS-IT-TOF analysis and bioassay in vitro. In total, 55 constituents involving 15 monoterpene glycosides, 13 galloyl glucoses, seven gallic acid derivatives, four acetophenones, six flavonoids, and ten other compounds were characterized according to their UV absorptions, MS/MS fragmentation, and comparing with reference compounds. Interestingly, one new monoterpene, oxypaeoniflorgenin, was assigned by its MSn and UV patterns. Quantitative analysis demonstrated that gallic acid (2) mainly in leaves (5439.1 +/- 90.2 mu g/g), paeoniflorin (13) mainly in root bark (16,378.9 +/- 195.4 mu g/g), root core (17,130.2 +/- 125.9 mu g/g), and leaves (16,682.9 +/- 181.2 mu g/g), and benzoylpaeoniflorin (46) and paeonol (49) mainly in root bark (2360.1 +/- 41.4 and 10,142.3 +/- 252.5 mu g/g) and root core (2249.5 +/- 21.7 and 10,441.6 +/- 363.9 mu g/g) were the main constituents in P. suffruticosa. The chemical constituents of root bark and root core were similar and obviously different from the stems and leaves. All the five parts were assayed for their agonistic effect on four neurotransmitter receptors (MT1, MT2, 5-HT1A, and 5-HT2C) and antidiabetic potency on alpha-glucosidase, PTP1B, and DPPIV. Besides root bark, the root core, stems, and leaves were also revealed with fascinating activity in this study. The extraction rate of root bark (35.4% +/- 2.18) was two times higher than that of root core (18.4% +/- 0.81), supporting the traditional application. This investigation provides new perspectives for understanding the chemical divergence between different parts of P. suffruticosa and their comprehensive utilization.
关键词	Paeonia suffruticosa Chemical profiles LCMS-IT-TOF analyses Biological comparison Neurotransmitter receptors Enzyme inhibition
DOI	10.1016/j.indcrop.2019.112028
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000510521700014
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/70853
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Geng, Chang-An
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Yunnan Key Lab Nat Med Chem, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Pan, Yang,Gao, Zhen,Huang, Xiao-Yan,et al. Chemical and biological comparison of different parts of Paeonia suffruticosa (Mudan) based on LCMS-IT-TOF and multi-evaluation in vitro[J]. INDUSTRIAL CROPS AND PRODUCTS,2020,144:9.
APA	Pan, Yang,Gao, Zhen,Huang, Xiao-Yan,Chen, Ji-Jun,&Geng, Chang-An.(2020).Chemical and biological comparison of different parts of Paeonia suffruticosa (Mudan) based on LCMS-IT-TOF and multi-evaluation in vitro.INDUSTRIAL CROPS AND PRODUCTS,144,9.
MLA	Pan, Yang,et al."Chemical and biological comparison of different parts of Paeonia suffruticosa (Mudan) based on LCMS-IT-TOF and multi-evaluation in vitro".INDUSTRIAL CROPS AND PRODUCTS 144(2020):9.