| Bioinformatics analysis of differentially expressed genes in hepatocellular carcinoma cells exposed to Swertiamarin | |
Tang, Haoran1; Ke, Yang2; Ren, Zongfang3; Lei, Xuefen4; Xiao, Shufeng2; Bao, Tianhao5; Shi, Zhitian2; Zou, Renchao2; Wu, Tiangen2; Zhou, Jian2; Geng, Chang-An6 ; Wang, Lin2; Chen, Jijun6
| |
| 通讯作者 | Wang, Lin(linwang0705@126.com) ; Chen, Jijun(chenjj@mail.kib.ac.cn) |
| 2019 | |
| 发表期刊 | JOURNAL OF CANCER
 |
| ISSN | 1837-9664 |
| 卷号 | 10期号:26页码:6526-6534 |
| 摘要 | Aim: To explore gene expression profiling in hepatocellular carcinoma (HCC) cells exposed to swertiamarin. Methods: Cell viability, apoptosis and invasion were examined in HepG2 cells after swertiamarin treatment. Tumor growth of SK-Hep-1 cells xenografted in nude mice was monitored after swertiamarin treatment. Total RNA was isolated from HepG2 cells treated with swertiamarin for microarray analysis. The data of microarray were analyzed by bioinformatics. Results: Swertiamarin treatment decreased the viability and invasion while increased the apoptosis of HepG2 cells, and significantly inhibited the growth of SK-Hep-1 cells xenografted in nude mice. Pathway and biological process analysis of differentially expressed genes (DEGs) in swertiamarin treated HepG2 cells showed that PI3k-Akt was the most significant regulated pathway. 47 targets of swertiamarin were predicted by CGBVS while 21 targets were predicted by 3NN. Notably, 8 targets were predicted as the targets of swertiamarin by both programs, including two prominent targets JUN and STAT3. A large range of DEGs induced by swertiamarin could be regulated by JUN and STAT3. Conclusion: Swertiamarin treatment led to significant changes in the expression of a variety of genes that modulate cell survival, cell cycle progression, apoptosis, and invasion. Moreover, most of these genes can be clustered into pathway networks such as PI3K, JUN, STAT3, which are predicted targets of swertiamarin. Further confirmation of these targets will reveal the anti-tumor mechanisms of swertiamarin and facilitate the development of swertiamarin as a novel agent for cancer prevention and treatment. |
| 关键词 | swertiamarin microarray HepG2 cells hepatocellular cancer Jun Stat3 |
| DOI | 10.7150/jca.33666 |
| 收录类别 | SCI ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000512664800006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/70822 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Wang, Lin; Chen, Jijun |
| 作者单位 | 1.Kunming Med Univ, Dept Gastroenterol Surg, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China 2.Kunming Med Univ, Dept Hepatobiliary Surg, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China 3.Kunming Med Univ, Dept Crit Care Med, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China 4.Kunming Med Univ, Dept Oncol, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China 5.Kunming Med Univ, Mental Hlth Ctr, Kunming, Yunnan, Peoples R China 6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Haoran,Ke, Yang,Ren, Zongfang,et al. Bioinformatics analysis of differentially expressed genes in hepatocellular carcinoma cells exposed to Swertiamarin[J]. JOURNAL OF CANCER,2019,10(26):6526-6534. |
| APA | Tang, Haoran.,Ke, Yang.,Ren, Zongfang.,Lei, Xuefen.,Xiao, Shufeng.,...&Chen, Jijun.(2019).Bioinformatics analysis of differentially expressed genes in hepatocellular carcinoma cells exposed to Swertiamarin.JOURNAL OF CANCER,10(26),6526-6534. |
| MLA | Tang, Haoran,et al."Bioinformatics analysis of differentially expressed genes in hepatocellular carcinoma cells exposed to Swertiamarin".JOURNAL OF CANCER 10.26(2019):6526-6534. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Tang-2019-Bioinforma（2228KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论