| An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection | |
Song, Na1,2; Ma, Lan1; Wang, Weiguang3,4; Sun, Huanhuan1; Wang, Lei1 ; Baldwin, Ian T.5; Wu, Jinsong1
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| Corresponding Author | Wu, Jinsong(jinsongwu@mail.kib.ac.cn) |
| 2019-10-15 | |
| Source Publication | JOURNAL OF EXPERIMENTAL BOTANY
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| ISSN | 0022-0957 |
| Volume | 70Issue:20Pages:5895-5908 |
| Abstract | Capsidiol is a sesquiterpenoid phytoalexin produced in Nicotiana and Capsicum species in response to pathogen attack. Whether capsidiol plays a defensive role and how its biosynthesis is regulated in the wild tobacco Nicotiana attenuata when the plant is attacked by Alternaria alternata (tobacco pathotype), a notorious necrotrophic fungus causing brown spot disease, are unknown. Transcriptome analysis indicated that a metabolic switch to sesquiterpene biosynthesis occurred in young leaves of N. attenuata after A. alternata inoculation: many genes leading to sesquiterpene production were strongly up-regulated, including the capsidiol biosynthetic genes 5-epi-aristolochene synthase (EAS) and 5-epi-aristolochene hydroxylase (EAH). Consistently, the level of capsidiol was increased dramatically in young leaves after fungal inoculation, from not detectable in mock control to 50.68 +/- 3.10 mu g g(-1) fresh leaf at 3 d post-inoculation. Capsidiol-reduced or capsidiol-depleted plants, which were generated by silencing EAHs or EASs by virus-induced gene silencing, were more susceptible to the fungus. In addition, this sesquiterpene when purified from infected plants exhibited strong anti-fungal activities against A. alternata in vitro. Furthermore, an ERF2-like transcription factor was found to positively regulate capsidiol production and plant resistance through the direct transactivation of a capsidiol biosynthetic gene, EAS12. Taken together, our results demonstrate that capsidiol, a phytoalexin highly accumulated in N. attenuata plants in response to A. alternata infection, plays an important role in pathogen resistance independent of jasmonate and ethylene signaling pathways, and its biosynthesis is transcriptionally regulated by an ERF2-like transcription factor. |
| Keyword | 5-epi-Aristolochene hydroxylase (EAH) 5-epi-aristolochene synthase (EAS) ethylene responsive factor Nicotiana attenuata phytoalexin sesquiterpene virus induced gene silencing (VIGS) |
| DOI | 10.1093/jxb/erz327 |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| WOS ID | WOS:000503022600030 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/70577 |
| Collection | 资源植物与生物技术所级重点实验室 |
| Corresponding Author | Wu, Jinsong |
| Affiliation | 1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Lanhei Rd 132, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Yunnan Minzu Univ, Key Lab Chem Ethn Med Resources, State Ethn Affairs Commiss, Kunming 650031, Yunnan, Peoples R China 4.Yunnan Minzu Univ, Minist Educ, Kunming 650031, Yunnan, Peoples R China 5.Max Planck Inst Chem Ecol, Dept Mol Ecol, D-07745 Jena, Germany |
| Recommended Citation GB/T 7714 | Song, Na,Ma, Lan,Wang, Weiguang,et al. An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(20):5895-5908. |
| APA | Song, Na.,Ma, Lan.,Wang, Weiguang.,Sun, Huanhuan.,Wang, Lei.,...&Wu, Jinsong.(2019).An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection.JOURNAL OF EXPERIMENTAL BOTANY,70(20),5895-5908. |
| MLA | Song, Na,et al."An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection".JOURNAL OF EXPERIMENTAL BOTANY 70.20(2019):5895-5908. |
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| Song-2019-An ERF2-li（3444KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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