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An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection
Song, Na1,2; Ma, Lan1; Wang, Weiguang3,4; Sun, Huanhuan1; Wang, Lei1; Baldwin, Ian T.5; Wu, Jinsong1
Corresponding AuthorWu, Jinsong(jinsongwu@mail.kib.ac.cn)
2019-10-15
Source PublicationJOURNAL OF EXPERIMENTAL BOTANY
ISSN0022-0957
Volume70Issue:20Pages:5895-5908
AbstractCapsidiol is a sesquiterpenoid phytoalexin produced in Nicotiana and Capsicum species in response to pathogen attack. Whether capsidiol plays a defensive role and how its biosynthesis is regulated in the wild tobacco Nicotiana attenuata when the plant is attacked by Alternaria alternata (tobacco pathotype), a notorious necrotrophic fungus causing brown spot disease, are unknown. Transcriptome analysis indicated that a metabolic switch to sesquiterpene biosynthesis occurred in young leaves of N. attenuata after A. alternata inoculation: many genes leading to sesquiterpene production were strongly up-regulated, including the capsidiol biosynthetic genes 5-epi-aristolochene synthase (EAS) and 5-epi-aristolochene hydroxylase (EAH). Consistently, the level of capsidiol was increased dramatically in young leaves after fungal inoculation, from not detectable in mock control to 50.68 +/- 3.10 mu g g(-1) fresh leaf at 3 d post-inoculation. Capsidiol-reduced or capsidiol-depleted plants, which were generated by silencing EAHs or EASs by virus-induced gene silencing, were more susceptible to the fungus. In addition, this sesquiterpene when purified from infected plants exhibited strong anti-fungal activities against A. alternata in vitro. Furthermore, an ERF2-like transcription factor was found to positively regulate capsidiol production and plant resistance through the direct transactivation of a capsidiol biosynthetic gene, EAS12. Taken together, our results demonstrate that capsidiol, a phytoalexin highly accumulated in N. attenuata plants in response to A. alternata infection, plays an important role in pathogen resistance independent of jasmonate and ethylene signaling pathways, and its biosynthesis is transcriptionally regulated by an ERF2-like transcription factor.
Keyword5-epi-Aristolochene hydroxylase (EAH) 5-epi-aristolochene synthase (EAS) ethylene responsive factor Nicotiana attenuata phytoalexin sesquiterpene virus induced gene silencing (VIGS)
DOI10.1093/jxb/erz327
Indexed BySCI ; SCI
Language英语
WOS IDWOS:000503022600030
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/70577
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorWu, Jinsong
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Lanhei Rd 132, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Yunnan Minzu Univ, Key Lab Chem Ethn Med Resources, State Ethn Affairs Commiss, Kunming 650031, Yunnan, Peoples R China
4.Yunnan Minzu Univ, Minist Educ, Kunming 650031, Yunnan, Peoples R China
5.Max Planck Inst Chem Ecol, Dept Mol Ecol, D-07745 Jena, Germany
Recommended Citation
GB/T 7714
Song, Na,Ma, Lan,Wang, Weiguang,et al. An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(20):5895-5908.
APA Song, Na.,Ma, Lan.,Wang, Weiguang.,Sun, Huanhuan.,Wang, Lei.,...&Wu, Jinsong.(2019).An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection.JOURNAL OF EXPERIMENTAL BOTANY,70(20),5895-5908.
MLA Song, Na,et al."An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection".JOURNAL OF EXPERIMENTAL BOTANY 70.20(2019):5895-5908.
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