| Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance | |
Yu, Lujun1; Chen, Qnfang1; Peng, Yujun1; Xie, Lijuan1; Liu, Di1; Han, Muqian1,2; Chen, Feng3; Xiao, Shi1; Huang, Junchao4 ; Li, Juan1,2
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| Corresponding Author | Huang, Junchao(huangjc@mail.kib.ac.cn) ; Li, Juan(adalee619@163.com) |
| 2019-11-13 | |
| Source Publication | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| ISSN | 0021-8561 |
| Volume | 67Issue:45Pages:12590-12598 |
| Abstract | Carotenoids play key roles in photosynthesis and photoprotection. Few multicellular plants produce the ketocarotenoid astaxanthin, a strong antioxidant; however, Arabidopsis thaliana lines overexpressing the Chlamydomonas reinhardtii beta-carotene ketolase (CrBKT) accumulated high amounts of astaxanthin in the leaves. In this study, we investigated the changed regulation of key metabolic pathways and the tolerance of the engineered plants to biotic and abiotic stresses resulting from the heterologous expression of CrBKT. Transcriptome analysis identified 1633 and 1722 genes that were differentially expressed in the leaves and siliques, respectively, of CrBKT-overexpressing plants (line CR5) as compared to wild-type Arabidopsis. These genes were enriched in the carotenoid biosynthetic pathways, and plant hormone biosynthesis and signaling pathways. In particular, metabolic profiling showed that, as compared to the wild-type leaves and siliques, overexpression of CrBKT increased the levels of most amino acids, but decreased the contents of sugars and carbohydrates. Furthermore, CR5 plants had lower sensitivity to abscisic acid (ABA) and increased tolerance to oxidative stress. CRS plants also exhibited enhanced resistance to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Our study provides insight into the regulation of carotenoids and the related pathways, which may be involved in plant response to oxidative stress and pathogen infection. |
| Keyword | carotenoid astaxanthin oxidation ABA BKT pathogen |
| DOI | 10.1021/acs.jafc.9b04589 |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| WOS ID | WOS:000497263900029 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/70438 |
| Collection | 资源植物与生物技术所级重点实验室 |
| Corresponding Author | Huang, Junchao; Li, Juan |
| Affiliation | 1.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol & Guangdong Prov Key Lab, Guangzhou 510275, Guangdong, Peoples R China 2.Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China 3.Shenzhen Univ, Inst Adv Study, Shenzhen 518000, Guangdong, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China |
| Recommended Citation GB/T 7714 | Yu, Lujun,Chen, Qnfang,Peng, Yujun,et al. Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(45):12590-12598. |
| APA | Yu, Lujun.,Chen, Qnfang.,Peng, Yujun.,Xie, Lijuan.,Liu, Di.,...&Li, Juan.(2019).Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(45),12590-12598. |
| MLA | Yu, Lujun,et al."Arabidopsis thaliana Plants Engineered To Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.45(2019):12590-12598. |
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| Yu-2019-Arabidopsis （3381KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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