Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity
Chen, Juan1; Li, Jia-Mei1; Tang, Yan-Jing1; Xing, Yong-Mei1; Qiao, Peng2; Li, Yang1; Liu, Pei-Gui3; Guo, Shun-Xing1
Corresponding AuthorChen, Juan(kibchenjuan@126.com) ; Guo, Shun-Xing(sxguo1986@163.com)
2019-11-05
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume10Pages:14
AbstractIt is well known that the microbes associated with truffle fruiting bodies play a very important role during the truffle lifecycle. Tuber indicum, commonly called Chinese black truffle, is a species endemic to Eastern Asia and in the genus of Tuber. Here, we reported the bacterial communities of T. indicum from different geographical regions and described the bacterial diversity from three compartments (soil, ectomycorrhizae and ascocarps) of T. indicum using high-throughput sequencing combined tissue culture. The results revealed that Bradyrhizobium was the dominant genus in fruiting bodies of T. indicum from nine geographical sites in China, and the microbes in T. indicum ascocarps were influenced by geological locations and soil characteristics. More specific bacterial taxa were enriched in the fruiting bodies than in the ectomycorrhizae and soil. In addition, 60 cultural bacteria were isolated from T. indicum fruiting bodies (4 families, 24 genera), and Pseudomonas, Alcaligenes faecalis, Microbacterium, and Arthrobacter were dominant. One of 13 strains that have potential nitrogen-fixation activities was further verified by an acetylene reduction assay (ARA). Together, this research provides new and important data for better understanding of the interaction between truffle and associated microbe and the biology of truffle itself.
KeywordChinese black truffle bacterial composition geographical region high-throughput tissue culture nitrogen fixation activity
DOI10.3389/fmicb.2019.02515
Indexed BySCI ; SCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000498569900001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/70106
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorChen, Juan; Guo, Shun-Xing
Affiliation1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Key Lab Bioact Subst & Resources Utilizat Chinese, Minist Educ, Beijing, Peoples R China
2.Shandong Acad Agr Sci, Shandong Inst Sericulture, Yantai, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Juan,Li, Jia-Mei,Tang, Yan-Jing,et al. Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity[J]. FRONTIERS IN MICROBIOLOGY,2019,10:14.
APA Chen, Juan.,Li, Jia-Mei.,Tang, Yan-Jing.,Xing, Yong-Mei.,Qiao, Peng.,...&Guo, Shun-Xing.(2019).Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity.FRONTIERS IN MICROBIOLOGY,10,14.
MLA Chen, Juan,et al."Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity".FRONTIERS IN MICROBIOLOGY 10(2019):14.
Files in This Item: Download All
File Name/Size DocType Version Access License
Chen-2019-Chinese Bl(2412KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Juan]'s Articles
[Li, Jia-Mei]'s Articles
[Tang, Yan-Jing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Juan]'s Articles
[Li, Jia-Mei]'s Articles
[Tang, Yan-Jing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Juan]'s Articles
[Li, Jia-Mei]'s Articles
[Tang, Yan-Jing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Chen-2019-Chinese Black Truffle-Associated Bac.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.