Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity

doi:10.3389/fmicb.2019.02515

	Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity
	Chen, Juan 1; Li, Jia-Mei 1; Tang, Yan-Jing 1; Xing, Yong-Mei 1; Qiao, Peng 2; Li, Yang 1; Liu, Pei-Gui3 ; Guo, Shun-Xing 1
通讯作者	Chen, Juan(kibchenjuan@126.com) ; Guo, Shun-Xing(sxguo1986@163.com)
	2019-11-05
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	10 页码:14
摘要	It is well known that the microbes associated with truffle fruiting bodies play a very important role during the truffle lifecycle. Tuber indicum, commonly called Chinese black truffle, is a species endemic to Eastern Asia and in the genus of Tuber. Here, we reported the bacterial communities of T. indicum from different geographical regions and described the bacterial diversity from three compartments (soil, ectomycorrhizae and ascocarps) of T. indicum using high-throughput sequencing combined tissue culture. The results revealed that Bradyrhizobium was the dominant genus in fruiting bodies of T. indicum from nine geographical sites in China, and the microbes in T. indicum ascocarps were influenced by geological locations and soil characteristics. More specific bacterial taxa were enriched in the fruiting bodies than in the ectomycorrhizae and soil. In addition, 60 cultural bacteria were isolated from T. indicum fruiting bodies (4 families, 24 genera), and Pseudomonas, Alcaligenes faecalis, Microbacterium, and Arthrobacter were dominant. One of 13 strains that have potential nitrogen-fixation activities was further verified by an acetylene reduction assay (ARA). Together, this research provides new and important data for better understanding of the interaction between truffle and associated microbe and the biology of truffle itself.
关键词	Chinese black truffle bacterial composition geographical region high-throughput tissue culture nitrogen fixation activity
DOI	10.3389/fmicb.2019.02515
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000498569900001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/70106
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Chen, Juan; Guo, Shun-Xing
作者单位	1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Key Lab Bioact Subst & Resources Utilizat Chinese, Minist Educ, Beijing, Peoples R China 2.Shandong Acad Agr Sci, Shandong Inst Sericulture, Yantai, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Chen, Juan,Li, Jia-Mei,Tang, Yan-Jing,et al. Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity[J]. FRONTIERS IN MICROBIOLOGY,2019,10:14.
APA	Chen, Juan.,Li, Jia-Mei.,Tang, Yan-Jing.,Xing, Yong-Mei.,Qiao, Peng.,...&Guo, Shun-Xing.(2019).Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity.FRONTIERS IN MICROBIOLOGY,10,14.
MLA	Chen, Juan,et al."Chinese Black Truffle-Associated Bacterial Communities of Tuber indicum From Different Geographical Regions With Nitrogen Fixing Bioactivity".FRONTIERS IN MICROBIOLOGY 10(2019):14.

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