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Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens
He, Jianfang1,2; Li, Ping1,2; Huo, Heqiang3; Liu, Lina1,2; Tang, Ting1; He, Mingxia1; Huang, Junchao1; Liu, Li1
Corresponding AuthorLiu, Li(liulia@mail.kib.ac.cn)
2019-08-01
Source PublicationPLANT DIVERSITY
ISSN2096-2703
Volume41Issue:4Pages:266-274
AbstractHeat stress can restrict plant growth, development, and crop yield. As essential plant antioxidants, carotenoids play significant roles in plant stress resistance. beta-carotene hydroxylase (BHY) and beta-carotene ketolase (BKT), which catalyze the conversions of beta-carotene to zeaxanthin and beta-carotene to canthaxanthin, respectively, are key enzymes in the carotenoid biosynthetic pathway, but little is known about their potential functions in stress resistance. Here, we investigated the roles of beta-carotene hydroxylase and beta-carotene ketolase during heat stress in Physcomitrella patens through expressing a beta-carotene ketolase gene from Chlamydomonas reinhardtii (CrBKT) and a beta-carotene hydroxylase gene from Haematococcus pluvialis (HpBHY) in the moss P. patens. In transgenic moss expressing these genes, carotenoids content increased (especially lutein content), and heat stress tolerance increased, with reduced leafy tissue necrosis. To investigate the mechanism of this heat stress resistance, we measured various physiological indicators and found a lower malondialdehyde level, higher peroxidase and superoxide dismutase activities, and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress. These results demonstrate that CrBKT and HpBHY increase plant heat stress resistance through the antioxidant and damage repair metabolism, which is related to abscisic acid and salicylate signaling. Copyright (C) 2019 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V.
KeywordCarotenoids Heat stress Antioxidant Abscisic acid Physcomitrella patens
DOI10.1016/j.pld.2019.04.001
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000484805800007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/68772
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorLiu, Li
Affiliation1.Chinese Acad Sci, Yunnan Key Lab Wild Plant Resources, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Florida, Dept Environm Hort, Midflorida Res & Educ Ctr, Apopka, FL 32703 USA
Recommended Citation
GB/T 7714
He, Jianfang,Li, Ping,Huo, Heqiang,et al. Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens[J]. PLANT DIVERSITY,2019,41(4):266-274.
APA He, Jianfang.,Li, Ping.,Huo, Heqiang.,Liu, Lina.,Tang, Ting.,...&Liu, Li.(2019).Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens.PLANT DIVERSITY,41(4),266-274.
MLA He, Jianfang,et al."Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens".PLANT DIVERSITY 41.4(2019):266-274.
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