| Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens | |
He, Jianfang1,2; Li, Ping1,2; Huo, Heqiang3; Liu, Lina1,2; Tang, Ting1; He, Mingxia1 ; Huang, Junchao1; Liu, Li1
| |
| Corresponding Author | Liu, Li(liulia@mail.kib.ac.cn) |
| 2019-08-01 | |
| Source Publication | PLANT DIVERSITY
 |
| ISSN | 2096-2703 |
| Volume | 41Issue:4Pages:266-274 |
| Abstract | Heat stress can restrict plant growth, development, and crop yield. As essential plant antioxidants, carotenoids play significant roles in plant stress resistance. beta-carotene hydroxylase (BHY) and beta-carotene ketolase (BKT), which catalyze the conversions of beta-carotene to zeaxanthin and beta-carotene to canthaxanthin, respectively, are key enzymes in the carotenoid biosynthetic pathway, but little is known about their potential functions in stress resistance. Here, we investigated the roles of beta-carotene hydroxylase and beta-carotene ketolase during heat stress in Physcomitrella patens through expressing a beta-carotene ketolase gene from Chlamydomonas reinhardtii (CrBKT) and a beta-carotene hydroxylase gene from Haematococcus pluvialis (HpBHY) in the moss P. patens. In transgenic moss expressing these genes, carotenoids content increased (especially lutein content), and heat stress tolerance increased, with reduced leafy tissue necrosis. To investigate the mechanism of this heat stress resistance, we measured various physiological indicators and found a lower malondialdehyde level, higher peroxidase and superoxide dismutase activities, and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress. These results demonstrate that CrBKT and HpBHY increase plant heat stress resistance through the antioxidant and damage repair metabolism, which is related to abscisic acid and salicylate signaling. Copyright (C) 2019 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. |
| Keyword | Carotenoids Heat stress Antioxidant Abscisic acid Physcomitrella patens |
| DOI | 10.1016/j.pld.2019.04.001 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000484805800007 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/68772 |
| Collection | 资源植物与生物技术所级重点实验室 |
| Corresponding Author | Liu, Li |
| Affiliation | 1.Chinese Acad Sci, Yunnan Key Lab Wild Plant Resources, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Florida, Dept Environm Hort, Midflorida Res & Educ Ctr, Apopka, FL 32703 USA |
| Recommended Citation GB/T 7714 | He, Jianfang,Li, Ping,Huo, Heqiang,et al. Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens[J]. PLANT DIVERSITY,2019,41(4):266-274. |
| APA | He, Jianfang.,Li, Ping.,Huo, Heqiang.,Liu, Lina.,Tang, Ting.,...&Liu, Li.(2019).Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens.PLANT DIVERSITY,41(4),266-274. |
| MLA | He, Jianfang,et al."Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens".PLANT DIVERSITY 41.4(2019):266-274. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| He-2019-Heterologous（1635KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment