Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens

doi:10.1016/j.pld.2019.04.001

	Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens
	He, Jianfang 1,2; Li, Ping 1,2; Huo, Heqiang 3; Liu, Lina 1,2; Tang, Ting 1; He, Mingxia1 ; Huang, Junchao1 ; Liu, Li1
通讯作者	Liu, Li(liulia@mail.kib.ac.cn)
	2019-08-01
发表期刊	PLANT DIVERSITY
ISSN	2096-2703
卷号	41 期号:4 页码:266-274
摘要	Heat stress can restrict plant growth, development, and crop yield. As essential plant antioxidants, carotenoids play significant roles in plant stress resistance. beta-carotene hydroxylase (BHY) and beta-carotene ketolase (BKT), which catalyze the conversions of beta-carotene to zeaxanthin and beta-carotene to canthaxanthin, respectively, are key enzymes in the carotenoid biosynthetic pathway, but little is known about their potential functions in stress resistance. Here, we investigated the roles of beta-carotene hydroxylase and beta-carotene ketolase during heat stress in Physcomitrella patens through expressing a beta-carotene ketolase gene from Chlamydomonas reinhardtii (CrBKT) and a beta-carotene hydroxylase gene from Haematococcus pluvialis (HpBHY) in the moss P. patens. In transgenic moss expressing these genes, carotenoids content increased (especially lutein content), and heat stress tolerance increased, with reduced leafy tissue necrosis. To investigate the mechanism of this heat stress resistance, we measured various physiological indicators and found a lower malondialdehyde level, higher peroxidase and superoxide dismutase activities, and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress. These results demonstrate that CrBKT and HpBHY increase plant heat stress resistance through the antioxidant and damage repair metabolism, which is related to abscisic acid and salicylate signaling. Copyright (C) 2019 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V.
关键词	Carotenoids Heat stress Antioxidant Abscisic acid Physcomitrella patens
DOI	10.1016/j.pld.2019.04.001
收录类别	SCI
语种	英语
WOS记录号	WOS:000484805800007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/68772
专题	资源植物与生物技术所级重点实验室
通讯作者	Liu, Li
作者单位	1.Chinese Acad Sci, Yunnan Key Lab Wild Plant Resources, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Florida, Dept Environm Hort, Midflorida Res & Educ Ctr, Apopka, FL 32703 USA
推荐引用方式 GB/T 7714	He, Jianfang,Li, Ping,Huo, Heqiang,et al. Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens[J]. PLANT DIVERSITY,2019,41(4):266-274.
APA	He, Jianfang.,Li, Ping.,Huo, Heqiang.,Liu, Lina.,Tang, Ting.,...&Liu, Li.(2019).Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens.PLANT DIVERSITY,41(4),266-274.
MLA	He, Jianfang,et al."Heterologous expression of HpBHY and CrBKT increases heat tolerance in Physcomitrella patens".PLANT DIVERSITY 41.4(2019):266-274.

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