Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean
Liu, Chongxi1,2; Zhuang, Xiaoxin1; Yu, Zhiyin1,2; Wang, Zhiyan2; Wang, Yongjiang2; Guo, Xiaowei1,2; Xiang, Wensheng1,3; Huang, Shengxiong2
Corresponding AuthorXiang, Wensheng(xiangwensheng@neau.edu.cn) ; Huang, Shengxiong(sxhuang@mail.kib.ac.cn)
2019-08-01
Source PublicationMICROORGANISMS
Volume7Issue:8Pages:15
AbstractThe present study was conducted to examine the influence of a pathogen Sclerotinia sclerotiorum (Lib.) de Bary on the actinobacterial community associated with the soybean roots. A total of 70 endophytic actinobacteria were isolated from the surface-sterilized roots of either healthy or diseased soybeans, and they were distributed under 14 genera. Some rare genera, including Rhodococcus, Kribbella, Glycomyces, Saccharothrix, Streptosporangium and Cellulosimicrobium, were endemic to the diseased samples, and the actinobacterial community was more diverse in the diseased samples compared with that in the heathy samples. Culture-independent analysis of root-associated actinobacterial community using the high-throughput sequencing approach also showed similar results. Four Streptomyces strains that were significantly abundant in the diseased samples exhibited strong antagonistic activity with the inhibition percentage of 54.1-87.6%. A bioactivity-guided approach was then employed to isolate and determine the chemical identity of antifungal constituents derived from the four strains. One new maremycin analogue, together with eight known compounds, were detected. All compounds showed significantly antifungal activity against S. sclerotiorum with the 50% inhibition (EC50) values of 49.14-0.21 mg/L. The higher actinobacterial diversity and more antifungal strains associated with roots of diseased plants indicate a possible role of the root-associated actinobacteria in natural defense against phytopathogens. Furthermore, these results also suggest that the root of diseased plant may be a potential reservoir of actinobacteria producing new agroactive compounds.
KeywordSclerotinia sclerotiorum (Lib ) de Bary diseased soybean root antifungal activity actinobacterial community new agroactive compounds
DOI10.3390/microorganisms7080243
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000482956300042
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/68610
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorXiang, Wensheng; Huang, Shengxiong
Affiliation1.Northeast Agr Univ, Heilongjiang Prov Key Lab Agr Microbiol, Harbin 150030, Heilongjiang, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
Recommended Citation
GB/T 7714
Liu, Chongxi,Zhuang, Xiaoxin,Yu, Zhiyin,et al. Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean[J]. MICROORGANISMS,2019,7(8):15.
APA Liu, Chongxi.,Zhuang, Xiaoxin.,Yu, Zhiyin.,Wang, Zhiyan.,Wang, Yongjiang.,...&Huang, Shengxiong.(2019).Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean.MICROORGANISMS,7(8),15.
MLA Liu, Chongxi,et al."Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean".MICROORGANISMS 7.8(2019):15.
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