Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway
Li, Cong1; Xu, Huanli1; Chen, Xiaoliang2; Chen, Jijun3; Li, Xiao1; Qiao, Gan1; Tian, Ye1; Yuan, Ru1; Su, Shuonan1; Liu, Xiaohui1; Lin, Xiukun1
Corresponding AuthorLin, Xiukun(xiukunlin@126.com)
2019-07-01
Source PublicationPHYTOTHERAPY RESEARCH
ISSN0951-418X
Volume33Issue:7Pages:1794-1804
AbstractCloves (Syzygium aromaticum), a traditional Chinese medicinal herb, displays broad biological activity. In the present study, the aqueous extract of clove (AEC) was prepared, and its anticancer affects were studied. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra-zolium (MTS) analysis revealed that AEC was able to inhibit cancer cell growth in vitro on several cancer cell lines; the IC50 is around 150 mu g/ml for human pancreatic ASPC-1 and human colon HT-29 cancer cells. Treatment of the cancer cells with AEC also diminished the colony formation significantly in both human pancreatic ASPC-1 cancer cells and human colon HT-29 cancer cells. In vivo study revealed that AEC inhibited the tumor growth significantly in HT-29 xenograft mice model. Transmission electron microscope, flow cytometry assay, and fluorescence microscope analysis confirmed that AEC is capable of inducing cell autophagy. Further study showed that AMPK/ULK pathway plays an important role in AEC-induced autophagy in cancer cells. Analysis of AEC components was performed by liquid chromatograph mass spectrometer approach, and more than nine constitutes were identified in AEC fraction. The study provides evidence that AEC has potential to be developed as a novel anticancer agent or as an adjuvant in cancer chemotherapy.
KeywordAMPK anticancer aqueous extract of clove autophagy traditional Chinese medicine
DOI10.1002/ptr.6367
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000477912800005
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/68127
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorLin, Xiukun
Affiliation1.Capital Med Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100069, Peoples R China
2.Shanxi Datong Univ, Basic Med Sch, Datong, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Li, Cong,Xu, Huanli,Chen, Xiaoliang,et al. Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway[J]. PHYTOTHERAPY RESEARCH,2019,33(7):1794-1804.
APA Li, Cong.,Xu, Huanli.,Chen, Xiaoliang.,Chen, Jijun.,Li, Xiao.,...&Lin, Xiukun.(2019).Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway.PHYTOTHERAPY RESEARCH,33(7),1794-1804.
MLA Li, Cong,et al."Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway".PHYTOTHERAPY RESEARCH 33.7(2019):1794-1804.
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