| Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway | |
Li, Cong1; Xu, Huanli1; Chen, Xiaoliang2; Chen, Jijun3 ; Li, Xiao1; Qiao, Gan1; Tian, Ye1; Yuan, Ru1; Su, Shuonan1; Liu, Xiaohui1; Lin, Xiukun1
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| 通讯作者 | Lin, Xiukun(xiukunlin@126.com) |
| 2019-07-01 | |
| 发表期刊 | PHYTOTHERAPY RESEARCH
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| ISSN | 0951-418X |
| 卷号 | 33期号:7页码:1794-1804 |
| 摘要 | Cloves (Syzygium aromaticum), a traditional Chinese medicinal herb, displays broad biological activity. In the present study, the aqueous extract of clove (AEC) was prepared, and its anticancer affects were studied. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra-zolium (MTS) analysis revealed that AEC was able to inhibit cancer cell growth in vitro on several cancer cell lines; the IC50 is around 150 mu g/ml for human pancreatic ASPC-1 and human colon HT-29 cancer cells. Treatment of the cancer cells with AEC also diminished the colony formation significantly in both human pancreatic ASPC-1 cancer cells and human colon HT-29 cancer cells. In vivo study revealed that AEC inhibited the tumor growth significantly in HT-29 xenograft mice model. Transmission electron microscope, flow cytometry assay, and fluorescence microscope analysis confirmed that AEC is capable of inducing cell autophagy. Further study showed that AMPK/ULK pathway plays an important role in AEC-induced autophagy in cancer cells. Analysis of AEC components was performed by liquid chromatograph mass spectrometer approach, and more than nine constitutes were identified in AEC fraction. The study provides evidence that AEC has potential to be developed as a novel anticancer agent or as an adjuvant in cancer chemotherapy. |
| 关键词 | AMPK anticancer aqueous extract of clove autophagy traditional Chinese medicine |
| DOI | 10.1002/ptr.6367 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000477912800005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/68127 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Lin, Xiukun |
| 作者单位 | 1.Capital Med Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100069, Peoples R China 2.Shanxi Datong Univ, Basic Med Sch, Datong, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Cong,Xu, Huanli,Chen, Xiaoliang,et al. Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway[J]. PHYTOTHERAPY RESEARCH,2019,33(7):1794-1804. |
| APA | Li, Cong.,Xu, Huanli.,Chen, Xiaoliang.,Chen, Jijun.,Li, Xiao.,...&Lin, Xiukun.(2019).Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway.PHYTOTHERAPY RESEARCH,33(7),1794-1804. |
| MLA | Li, Cong,et al."Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway".PHYTOTHERAPY RESEARCH 33.7(2019):1794-1804. |
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| li2019.pdf（1218KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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