| Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass | |
| Zhang, Rui1,2; Li, Na1,2; Xu, Shujing1,2; Han, Xiaowei1,2; Li, Chunyan1,2; Wei, Xin3; Liu, Yu1,2; Tu, Tao4; Tang, Xianghua1,2; Zhou, Junpei1,2; Huang, Zunxi1,2 | |
| 通讯作者 | Zhou, Junpei(junpeizhou@ynnu.edu.cn) ; Huang, Zunxi(huangzunxi@163.com) |
| 2019-03-20 | |
| 发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| ISSN | 0021-8561 |
| 卷号 | 67期号:11页码:3220-3228 |
| 摘要 | beta-1,2-Xylosidase activity has not been recorded as an EC subsubclass. In this study, phylogenetic analysis and multiple sequence alignments revealed that characterized beta-xylosidases of glycoside hydrolase family (GH) 39 were classified into the same subgroup with conserved amino acid residue positions participating in substrate recognition. Protein-ligand docking revealed that seven of these positions were probably essential to bind xylose-glucose, which is linked by a beta-1,2-glycosidic bond. Amino acid residues in five of the seven positions are invariant, while those in two of the seven positions are variable with low frequency. Both the wild-type beta-xylosidase rJB13GH39 and its mutants with mutation at the two positions exhibited beta-1,2-xylosidase activity, as they hydrolyzed o-nitrophenyl-beta-D-xylopyranoside and transformed notoginsenosides R-1 and R-2 to ginsenosides Rg(1) and Rh-1, respectively. The results suggest that all of these characterized GH 39 beta-xylosidases probably show beta-1,2-xylosidase activity, which should be assigned an EC number with these beta-xylosidases as representatives. |
| 关键词 | beta-xylosidase beta-1,2-glycosidic bond enzyme commission notoginsenosides glycoside hydrolase family 39 |
| DOI | 10.1021/acs.jafc.9b00027 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000462260500017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/67665 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Zhou, Junpei; Huang, Zunxi |
| 作者单位 | 1.Yunnan Normal Univ, Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Minist Educ, Kunming 650500, Yunnan, Peoples R China 2.Yunnan Normal Univ, Coll Life Sci, 768 Juxian St, Kunming 650500, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Rui,Li, Na,Xu, Shujing,et al. Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(11):3220-3228. |
| APA | Zhang, Rui.,Li, Na.,Xu, Shujing.,Han, Xiaowei.,Li, Chunyan.,...&Huang, Zunxi.(2019).Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(11),3220-3228. |
| MLA | Zhang, Rui,et al."Glycoside Hydrolase Family 39 beta-Xylosidases Exhibit beta-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.11(2019):3220-3228. |
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| Zhang-2019-Glycoside（6317KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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