Complete loss of RNA editing from the plastid genome and most highly expressed mitochondrial genes of Welwitschia mirabilis

doi:10.1007/s11427-018-9450-1

	Complete loss of RNA editing from the plastid genome and most highly expressed mitochondrial genes of Welwitschia mirabilis
	Fan, Weishu 1,2,3; Guo, Wenhu 2,4; Funk, Lexis 2,4; Mower, Jeffrey P.2,3; Zhu, Andan 1
通讯作者	Mower, Jeffrey P.(jpmower@unl.edu) ; Zhu, Andan(zhuandan@mail.kib.ac.cn)
	2019-04-01
发表期刊	SCIENCE CHINA-LIFE SCIENCES
ISSN	1674-7305
卷号	62 期号:4 页码:498-506
摘要	Comparative genomics among gymnosperms suggested extensive loss of mitochondrial RNA editing sites from Welwitschia mirabilis based on predictive analysis. However, empirical or transcriptome data to confirm this massive loss event are lacking, and the potential mechanisms of RNA site loss are unclear. By comparing genomic sequences with transcriptomic and reverse-transcription PCR sequencing data, we performed a comprehensive analysis of the pattern of RNA editing in the mitochondrial and plastid genomes (mitogenome and plastome, respectively) of W. mirabilis and a second gymnosperm, Ginkgo biloba. For W. mirabilis, we found only 99 editing sites located in 13 protein-coding genes in the mitogenome and a complete loss of RNA editing from the plastome. The few genes having high editing frequency in the Welwitschia mitogenome showed a strong negative correlation with gene expression level. Comparative analyses with G. biloba, containing 1,405 mitochondrial and 345 plastid editing sites, revealed that the editing loss from W. mirabilis is mainly due to the substitution of editable cytidines to thymidines at the genomic level, which could be caused by retroprocessing. Our result is the first study to uncover massive editing loss from both the mitogenome and plastome in a single genus. Furthermore, our results suggest that gene expression level and retroprocessing both contributed to the evolution of RNA editing in plant organellar genomes.
关键词	RNA editing massive loss expression levels organelle genomes Welwitschia
DOI	10.1007/s11427-018-9450-1
收录类别	SCI
语种	英语
WOS记录号	WOS:000464901000006
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/67604
专题	中国西南野生生物种质资源库
通讯作者	Mower, Jeffrey P.; Zhu, Andan
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 2.Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA 3.Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA 4.Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA
推荐引用方式 GB/T 7714	Fan, Weishu,Guo, Wenhu,Funk, Lexis,et al. Complete loss of RNA editing from the plastid genome and most highly expressed mitochondrial genes of Welwitschia mirabilis[J]. SCIENCE CHINA-LIFE SCIENCES,2019,62(4):498-506.
APA	Fan, Weishu,Guo, Wenhu,Funk, Lexis,Mower, Jeffrey P.,&Zhu, Andan.(2019).Complete loss of RNA editing from the plastid genome and most highly expressed mitochondrial genes of Welwitschia mirabilis.SCIENCE CHINA-LIFE SCIENCES,62(4),498-506.
MLA	Fan, Weishu,et al."Complete loss of RNA editing from the plastid genome and most highly expressed mitochondrial genes of Welwitschia mirabilis".SCIENCE CHINA-LIFE SCIENCES 62.4(2019):498-506.

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