Physalis peruviana-Derived 4 beta-Hydroxywithanolide E, a Novel Antagonist of Wnt Signaling, Inhibits Colorectal Cancer In Vitro and In Vivo

doi:10.3390/molecules24061146

	Physalis peruviana-Derived 4 beta-Hydroxywithanolide E, a Novel Antagonist of Wnt Signaling, Inhibits Colorectal Cancer In Vitro and In Vivo
	Ye, Zhen-Nan 1,2,3; Yuan, Feng 1,2; Liu, Jie-Qing 1; Peng, Xing-Rong 1; An, Tao1 ; Li, Xue1,2 ; Kong, Ling-Mei 1; Qiu, Ming-Hua1 ; Li, Yan1
通讯作者	Kong, Ling-Mei(konglingmei@mail.kib.ac.cn) ; Qiu, Ming-Hua(mhchiu@mail.kib.ac.cn) ; Li, Yan(liyanb@mail.kib.ac.cn)
	2019-03-22
发表期刊	MOLECULES
ISSN	1420-3049
卷号	24 期号:6 页码:13
摘要	Deregulation of the Wnt signaling pathway leads to colorectal cancer progression. Natural dietary compounds serve as promising candidates for development as chemopreventive agents by suppressing the Wnt/beta-catenin signaling pathway. Physalis peruviana-derived 4 beta HWE showed a significant inhibitory activity with a calculated IC50 of 0.09 mu M in a screening of novel inhibitors of Wnt signaling with the dual-luciferase reporter assay. This study investigated the anti-tumor effect of 4 beta HWE and the potential Wnt signaling inhibitory mechanism. Both the western blot analysis and immunofluorescence assay showed that 4 beta HWE promoted the phosphorylation and degradation of beta-catenin and the subsequent inhibition of its nuclear translocation to attenuate the endogenous Wnt target gene expression in colorectal cancer (CRC) cells. The cell viability assay indicated that 4 beta HWE preferentially inhibited the proliferation of CRC compared with CCD-841-CoN, a normal human colonic epithelial cell line. 4 beta HWE-mediated G0/G1 cell cycle arrest and apoptosis induction contributed to the suppression of the proliferation of CRC in the cell cycle and Annexin V-FITC/Propidium Iodide apoptosis analysis. Moreover, in vivo, 4 beta HWE dramatically inhibited tumor growth in HCT116 xenografts by attenuating the Wnt/beta-catenin signaling pathway. In conclusion, our study suggested that 4 beta HWE could be of potential use in anti-tumor agent development as a novel Wnt signaling inhibitor.
关键词	4 beta HWE Wnt signaling pathway beta-catenin colorectal cancer
DOI	10.3390/molecules24061146
收录类别	SCI
语种	英语
WOS记录号	WOS:000464290900008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/67595
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Kong, Ling-Mei; Qiu, Ming-Hua; Li, Yan
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jena Univ Hosp, Dept Biochem 2, D-07743 Jena, Germany
推荐引用方式 GB/T 7714	Ye, Zhen-Nan,Yuan, Feng,Liu, Jie-Qing,et al. Physalis peruviana-Derived 4 beta-Hydroxywithanolide E, a Novel Antagonist of Wnt Signaling, Inhibits Colorectal Cancer In Vitro and In Vivo[J]. MOLECULES,2019,24(6):13.
APA	Ye, Zhen-Nan.,Yuan, Feng.,Liu, Jie-Qing.,Peng, Xing-Rong.,An, Tao.,...&Li, Yan.(2019).Physalis peruviana-Derived 4 beta-Hydroxywithanolide E, a Novel Antagonist of Wnt Signaling, Inhibits Colorectal Cancer In Vitro and In Vivo.MOLECULES,24(6),13.
MLA	Ye, Zhen-Nan,et al."Physalis peruviana-Derived 4 beta-Hydroxywithanolide E, a Novel Antagonist of Wnt Signaling, Inhibits Colorectal Cancer In Vitro and In Vivo".MOLECULES 24.6(2019):13.

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