| Structure and heparanase inhibitory activity of a new glycosaminoglycan from the slug Limacus flavus | |
He, Zhicheng1,2; Zhou, Lutan1,2; Lin, Lisha1,2; Yin, Ronghua1; Zhao, Jinhua1
| |
| 通讯作者 | Yin, Ronghua(yinronghua@mail.kib.ac.cn) ; Zhao, Jinhua(zhao.jinhua@yahoo.com) |
| 2019-09-15 | |
| 发表期刊 | CARBOHYDRATE POLYMERS
 |
| ISSN | 0144-8617 |
| 卷号 | 220页码:176-184 |
| 摘要 | A new glycosaminoglycan (LF-GAG) was purified from the slug Limacus flavus. Its unique chemical structure and heparanase inhibitory activity were studied in this work. The native LF-GAG was composed of L-iduronic acid (LIdoA) and N-acetyl-D-glucosamine (D-GlcNAc), with a Mw of 22,700 Da. To elucidate the precise structure and structure-activity relationship, its deacetylation-deaminative depolymerized product (dLF-GAG) was prepared, and from which four oligosaccharides were purified. Combining the NMR spectral analysis of LF-GAG and its derived oligosaccharides, the structure of LF-GAG was deduced to be -4)-L-IdoA2R-(a1,4)-D-GlcNAc-(a1-, in which R was -OH (similar to 80%) or -OSO3 -(similar to 20%). Bioactivity assays showed that LF-GAG could potently inhibit human heparanase (IC50, 0.10 mu M). dLF-GAG and LF-3 were less potent but also active for heparanase inhibition. Structure-activity relationship analysis indicated that the chain length and sulfate substitution of LF-GAG are essential for its heparanase inhibitory activity. |
| 关键词 | Slug Glycosaminoglycan Chemical structure Depolymerization Heparanase inhibition |
| DOI | 10.1016/j.carbpol.2019.05.066 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000470825500022 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/67324 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Yin, Ronghua; Zhao, Jinhua |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Zhicheng,Zhou, Lutan,Lin, Lisha,et al. Structure and heparanase inhibitory activity of a new glycosaminoglycan from the slug Limacus flavus[J]. CARBOHYDRATE POLYMERS,2019,220:176-184. |
| APA | He, Zhicheng,Zhou, Lutan,Lin, Lisha,Yin, Ronghua,&Zhao, Jinhua.(2019).Structure and heparanase inhibitory activity of a new glycosaminoglycan from the slug Limacus flavus.CARBOHYDRATE POLYMERS,220,176-184. |
| MLA | He, Zhicheng,et al."Structure and heparanase inhibitory activity of a new glycosaminoglycan from the slug Limacus flavus".CARBOHYDRATE POLYMERS 220(2019):176-184. |
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| He-2019-Structure an（1875KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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