Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining
Li, Xin-Lin1; Xu, Yu-Xing5; Li, Yuan3; Zhang, Rui1; Hu, Tian-Yuan1; Su, Ping3; Zhou, Man2; Tang, Ting2; Zeng, Ying4; Yang, Yan-Long2; Gao, Wei1,6
Corresponding AuthorYang, Yan-Long(ylyang@lzu.edu.cn) ; Gao, Wei(weigao@ccmu.edu.cn)
2019-07-01
Source PublicationFUNGAL GENETICS AND BIOLOGY
ISSN1087-1845
Volume128Pages:36-42
AbstractBasidiomycete fungi are a rich source of bioactive diterpenoid secondary metabolites. However, compared with the large number of diterpene synthases (di-TPSs) identified in plants and ascomycete fungi, only three di-TPSs have been described from basidiomycete fungi. Large scale genome sequencing projects combined with the development of synthetic biology techniques now has enabled the rapidly discovery and characterization of di-TPSs from basidiomycete fungi. In this study, we discovered and functionally characterized four di-TPSs from 220 genome sequenced basidiomycete fungi by a combined strategy of genomic data mining, phylogenetic analysis and fast products characterization with synthetic biology techniques. Among them, SteTC1 of Stereum histurum was characterized as the first fungal cembrane diterpene synthase; PunTC of Punctularia strigosozonata and SerTC of Serpula lacryrnans were characterized as ent-kauran-16 alpha-ol synthase and DenTC3 of Dentipellis sp was characterized as a cyathane synthase. Our results provide opportunities for the discovery of new diterpenoids from basidiomycete fungi by genome mining.
KeywordBasidiomycete Ditepene synthase Genome mining Cembrane diterpene (-)-cyatha-3,12-diene Ent-kauran-16 alpha-ol
DOI10.1016/j.fgb.2019.03.007
Indexed BySCI
Language英语
WOS IDWOS:000471736900005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/67238
Collection植物化学与西部植物资源持续利用国家重点实验室
资源植物与生物技术所级重点实验室
Corresponding AuthorYang, Yan-Long; Gao, Wei
Affiliation1.Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China
2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
3.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
6.Capital Med Univ, Sch Pharmaceut Sci, Beijing 100069, Peoples R China
Recommended Citation
GB/T 7714
Li, Xin-Lin,Xu, Yu-Xing,Li, Yuan,et al. Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining[J]. FUNGAL GENETICS AND BIOLOGY,2019,128:36-42.
APA Li, Xin-Lin.,Xu, Yu-Xing.,Li, Yuan.,Zhang, Rui.,Hu, Tian-Yuan.,...&Gao, Wei.(2019).Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining.FUNGAL GENETICS AND BIOLOGY,128,36-42.
MLA Li, Xin-Lin,et al."Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining".FUNGAL GENETICS AND BIOLOGY 128(2019):36-42.
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