Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments

doi:10.3390/md17040195

	Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments
	Guan, Ruowei 1; Peng, Yuan 1; Zhou, Lutan 2,3; Zheng, Wenqi 1; Liu, Xixi 1; Wang, Pin 1; Yuan, Qingxia 1; Gao, Na 1; Zhao, Longyan1 ; Zhao, Jinhua1,2
通讯作者	Gao, Na(gn2008.happy@163.com) ; Zhao, Longyan(zhaolongyan@mail.scuec.edu.cn) ; Zhao, Jinhua(zhaojinhua@mail.kib.ac.cn)
	2019-04-01
发表期刊	MARINE DRUGS
ISSN	1660-3397
卷号	17 期号:4 页码:13
摘要	Apostichopus japonicus is one of the most economically important species in sea cucumber aquaculture in China. Fucosylated glycosaminoglycan from A. japonicus (AjFG) has shown multiple pharmacological activities. However, results from studies on the structure of AjFG are still controversial. In this study, the deaminative depolymerization method that is glycosidic bond-selective was used to prepare the depolymerized products from AjFG (dAjFG), and then a series of purified oligosaccharide fragments such as tri-, hexa-, nona-, and dodecasaccharides were obtained from dAjFG by gel permeation chromatography. The 1D/2D NMR and ESI-MS spectrometry analyses showed that these oligosaccharides had the structural formula of l-FucS-1,3-d-GlcA-1,3-{d-GalNAc(4S6S)-1,4-[l-FucS-1,3-]d-GlcA-1,3-}(n)-d-anTal-diol(4S6S) (n = 0, 1, 2, 3; FucS represents Fuc(2S4S), Fuc(3S4S), or Fuc(4S)). Thus, the unambiguous structure of native AjFG can be rationally deduced: it had the backbone of {-4-d-GlcA-1,3-d-GalNAc(4S6S)-1-}(n), which is similar to chondroitin sulfate E, and each d-GlcA residue in the backbone was branched with a l-FucS monosaccharide at O-3. Bioactivity assays confirmed that dAjFG and nonasaccharides and dodecasaccharides from AjFG had potent anticoagulant activity by intrinsic FXase inhibition while avoiding side effects such as FXII activation and platelet aggregation.
关键词	Apostichopus japonicus fucosylated glycosaminoglycan oligosaccharide unambiguous structure anti-Fxase
DOI	10.3390/md17040195
收录类别	SCI
语种	英语
WOS记录号	WOS:000467307100002
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/66876
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Gao, Na; Zhao, Longyan; Zhao, Jinhua
作者单位	1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guan, Ruowei,Peng, Yuan,Zhou, Lutan,et al. Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments[J]. MARINE DRUGS,2019,17(4):13.
APA	Guan, Ruowei.,Peng, Yuan.,Zhou, Lutan.,Zheng, Wenqi.,Liu, Xixi.,...&Zhao, Jinhua.(2019).Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments.MARINE DRUGS,17(4),13.
MLA	Guan, Ruowei,et al."Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments".MARINE DRUGS 17.4(2019):13.

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