Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments
Guan, Ruowei1; Peng, Yuan1; Zhou, Lutan2,3; Zheng, Wenqi1; Liu, Xixi1; Wang, Pin1; Yuan, Qingxia1; Gao, Na1; Zhao, Longyan1; Zhao, Jinhua1,2
Corresponding AuthorGao, Na(gn2008.happy@163.com) ; Zhao, Longyan(zhaolongyan@mail.scuec.edu.cn) ; Zhao, Jinhua(zhaojinhua@mail.kib.ac.cn)
2019-04-01
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume17Issue:4Pages:13
AbstractApostichopus japonicus is one of the most economically important species in sea cucumber aquaculture in China. Fucosylated glycosaminoglycan from A. japonicus (AjFG) has shown multiple pharmacological activities. However, results from studies on the structure of AjFG are still controversial. In this study, the deaminative depolymerization method that is glycosidic bond-selective was used to prepare the depolymerized products from AjFG (dAjFG), and then a series of purified oligosaccharide fragments such as tri-, hexa-, nona-, and dodecasaccharides were obtained from dAjFG by gel permeation chromatography. The 1D/2D NMR and ESI-MS spectrometry analyses showed that these oligosaccharides had the structural formula of l-FucS-1,3-d-GlcA-1,3-{d-GalNAc(4S6S)-1,4-[l-FucS-1,3-]d-GlcA-1,3-}(n)-d-anTal-diol(4S6S) (n = 0, 1, 2, 3; FucS represents Fuc(2S4S), Fuc(3S4S), or Fuc(4S)). Thus, the unambiguous structure of native AjFG can be rationally deduced: it had the backbone of {-4-d-GlcA-1,3-d-GalNAc(4S6S)-1-}(n), which is similar to chondroitin sulfate E, and each d-GlcA residue in the backbone was branched with a l-FucS monosaccharide at O-3. Bioactivity assays confirmed that dAjFG and nonasaccharides and dodecasaccharides from AjFG had potent anticoagulant activity by intrinsic FXase inhibition while avoiding side effects such as FXII activation and platelet aggregation.
KeywordApostichopus japonicus fucosylated glycosaminoglycan oligosaccharide unambiguous structure anti-Fxase
DOI10.3390/md17040195
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000467307100002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/66876
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorGao, Na; Zhao, Longyan; Zhao, Jinhua
Affiliation1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Guan, Ruowei,Peng, Yuan,Zhou, Lutan,et al. Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments[J]. MARINE DRUGS,2019,17(4):13.
APA Guan, Ruowei.,Peng, Yuan.,Zhou, Lutan.,Zheng, Wenqi.,Liu, Xixi.,...&Zhao, Jinhua.(2019).Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments.MARINE DRUGS,17(4),13.
MLA Guan, Ruowei,et al."Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments".MARINE DRUGS 17.4(2019):13.
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