Metabolic Activation of Myristicin and Its Role in Cellular Toxicity
Zhu, Xu1,2; Wang, Yi-Kun1,2; Yang, Xiao-Nan1,3; Xiao, Xue-Rong1; Zhang, Ting1,2; Yang, Xiu-Wei4; Qin, Hong-Bo1; Li, Fei1,5
Corresponding AuthorQin, Hong-Bo(qinhongbo@mail.kib.ac.cn) ; Li, Fei(lifeib@mail.kib.ac.cn)
2019-04-17
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
Volume67Issue:15Pages:4328-4336
AbstractMyristicin is widely distributed in spices and medicinal plants. The aim of this study was to explore the role of metabolic activation of myristicin in its potential toxicity through a metabolomic approach. The myristicin-N-acetylcysteine adduct was identified by comparing the metabolic maps of myristicin and 1'-hydroxymyristicin. The supplement of N-acetylcysteine could protect against the cytotoxicity of myristicin and 1'-hydroxymyristicin in primary mouse hepatocytes. When the depletion of intracellular N-acetylcysteine was pretreated with diethyl maleate in hepatocytes, the cytotoxicity induced by myristicin and 1'-hydroxymyristicin was deteriorated. It suggested that the N-acetylcysteine adduct resulting from myristicin bioactivation was closely associated with myristicin toxicity. Screening of human recombinant cytochrome P450s (CYPs) and treatment with CYP inhibitors revealed that CYP1A1 was mainly involved in the formation of 1'-hydroxymyristicin. Collectively, this study provided a global view of myristicin metabolism and identified the N-acetylcysteine adduct resulting from myristicin bioactivation, which could be used for understanding the mechanism of myristicin toxicity.
Keywordmyristicin 1 '-hydroxymyristicin metabolic activation metabolomics
DOI10.1021/acs.jafc.9b00893
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000465189400022
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/66349
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorQin, Hong-Bo; Li, Fei
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, States Key Lab Phytochem & Plant Resources West C, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Guangxi Bot Garden Med Plant, Guangxi Key Lab Med Resources Protect & Genet Imp, Nanning 530023, Guangxi, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
5.Jiangxi Univ Tradit Chinese Med, Nanchang 330004, Jiangxi, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Xu,Wang, Yi-Kun,Yang, Xiao-Nan,et al. Metabolic Activation of Myristicin and Its Role in Cellular Toxicity[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(15):4328-4336.
APA Zhu, Xu.,Wang, Yi-Kun.,Yang, Xiao-Nan.,Xiao, Xue-Rong.,Zhang, Ting.,...&Li, Fei.(2019).Metabolic Activation of Myristicin and Its Role in Cellular Toxicity.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(15),4328-4336.
MLA Zhu, Xu,et al."Metabolic Activation of Myristicin and Its Role in Cellular Toxicity".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.15(2019):4328-4336.
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