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Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like
Li, Jian1; Ma, Lan1; Zhang, Shuting1,2; Zuo, Cailian1; Song, Na1; Zhu, Shusheng3; Wu, Jinsong1
2019-04-01
Source PublicationPLANTA
ISSN0032-0935
Volume249Issue:4Pages:1229-1237
AbstractMain conclusionTranscriptome analysis revealed high expression of saponin biosynthetic genes may account for highly accumulated saponins in 3-year-oldPanax notoginsengroots andDSandCYP716A47-likewere functionally verified by transgenic tobacco.Panax notoginseng is a well-known traditional medical herb that contains bioactive compounds known as saponins. Three major dammarene-type triterpene saponins including R1, Rb1, and Rg1 were found to be highly accumulated in the roots of 3-year-old plants when compared to those of 1-year-old plants. However, the underlying cellular mechanism is poorly understood. In this study, transcriptome analysis revealed that most genes involved in saponin biosynthesis in P. notoginseng roots augmented during their growth periods. The analysis of the KEGG pathway indicated that the primary metabolism, cell growth, and differentiation were less active in the roots of 3-year-old plant; however, secondary metabolisms were enhanced, thus providing molecular evidence for the harvesting of P. notoginseng roots in the 3rd year of growth. Furthermore, the functional role of DS and CYP716A47-like, two of the candidate genes involved in saponin biosynthesis isolated from P. notoginseng, were verified via overexpression in cultivated tobacco. Approximately, 0.325 mu gg(-1) of dammarenediol-II and 0.320 mu gg(-1) of protopanaxadiol were recorded in the dry leaves of transgenic tobacco overexpressed with DS and both DS and CYP716A47-like, respectively. This study provides insights into the molecular mechanisms for saponin accumulation in P. notoginseng roots during its growth period and paves a promising way to produce dammarenediol-II and protopanaxadiol via transgenic techniques.
Keyword1-and 3-year-old Panax notoginseng Dammarene-type triterpene saponins RNA-seq Transgenic tobacco
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/65180
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorWu, Jinsong
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Lanhei Rd 132, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Key Lab Agrobiodivers & Pest Management, Educ Minist China, Kunming 650201, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Li, Jian,Ma, Lan,Zhang, Shuting,et al. Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like[J]. PLANTA,2019,249(4):1229-1237.
APA Li, Jian.,Ma, Lan.,Zhang, Shuting.,Zuo, Cailian.,Song, Na.,...&Wu, Jinsong.(2019).Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like.PLANTA,249(4),1229-1237.
MLA Li, Jian,et al."Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like".PLANTA 249.4(2019):1229-1237.
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