Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like

	Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like
	Li, Jian 1; Ma, Lan 1; Zhang, Shuting 1,2; Zuo, Cailian 1; Song, Na 1; Zhu, Shusheng 3; Wu, Jinsong 1
	2019-04-01
发表期刊	PLANTA
ISSN	0032-0935
卷号	249 期号:4 页码:1229-1237
摘要	Main conclusionTranscriptome analysis revealed high expression of saponin biosynthetic genes may account for highly accumulated saponins in 3-year-oldPanax notoginsengroots andDSandCYP716A47-likewere functionally verified by transgenic tobacco.Panax notoginseng is a well-known traditional medical herb that contains bioactive compounds known as saponins. Three major dammarene-type triterpene saponins including R1, Rb1, and Rg1 were found to be highly accumulated in the roots of 3-year-old plants when compared to those of 1-year-old plants. However, the underlying cellular mechanism is poorly understood. In this study, transcriptome analysis revealed that most genes involved in saponin biosynthesis in P. notoginseng roots augmented during their growth periods. The analysis of the KEGG pathway indicated that the primary metabolism, cell growth, and differentiation were less active in the roots of 3-year-old plant; however, secondary metabolisms were enhanced, thus providing molecular evidence for the harvesting of P. notoginseng roots in the 3rd year of growth. Furthermore, the functional role of DS and CYP716A47-like, two of the candidate genes involved in saponin biosynthesis isolated from P. notoginseng, were verified via overexpression in cultivated tobacco. Approximately, 0.325 mu gg(-1) of dammarenediol-II and 0.320 mu gg(-1) of protopanaxadiol were recorded in the dry leaves of transgenic tobacco overexpressed with DS and both DS and CYP716A47-like, respectively. This study provides insights into the molecular mechanisms for saponin accumulation in P. notoginseng roots during its growth period and paves a promising way to produce dammarenediol-II and protopanaxadiol via transgenic techniques.
关键词	1-and 3-year-old Panax notoginseng Dammarene-type triterpene saponins RNA-seq Transgenic tobacco
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/65180
专题	资源植物与生物技术所级重点实验室
通讯作者	Wu, Jinsong
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Lanhei Rd 132, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Key Lab Agrobiodivers & Pest Management, Educ Minist China, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Li, Jian,Ma, Lan,Zhang, Shuting,et al. Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like[J]. PLANTA,2019,249(4):1229-1237.
APA	Li, Jian.,Ma, Lan.,Zhang, Shuting.,Zuo, Cailian.,Song, Na.,...&Wu, Jinsong.(2019).Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like.PLANTA,249(4),1229-1237.
MLA	Li, Jian,et al."Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like".PLANTA 249.4(2019):1229-1237.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Li-2019-Transcriptom（1467KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载