IRE1-bZIP60 Pathway Is Required for Nicotiana attenuata Resistance to Fungal Pathogen Alternaria alternata

	IRE1-bZIP60 Pathway Is Required for Nicotiana attenuata Resistance to Fungal Pathogen Alternaria alternata
	Xu, Zhen 1; Song, Na 1,2; Ma, Lan 1; Wu, Jinsong 1
	2019-03-19
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	10 页码:10
摘要	As an endoplasmic reticulum (ER) stress sensor, inositol-requiring enzyme 1 (IRE1) splices the bZIP60 mRNA, and produces an active bZIP60 transcription factor that regulates genes involved in the unfolded protein response (UPR) during ER stresses. This IRE1-bZIP60 pathway is conserved in plant species and recently implicated in plant-pathogen interaction. However, it is unclear whether this IRE1-bZIP60 pathway is involved in Nicotiana attenuate resistance to necrotic fungal pathogen, Alternaria alternata. In this study, transcriptional levels of chaperone protein genes, including luminal binding protein (BiP), protein disulfide isomerase (PDI), calnexin 1-like (CNX 1-like), and calreticulin (CRT), and genes involved in IRE1-bZIP60 pathway, were all significantly induced in N. attenuate leaves after A. alternata inoculation. Silencing IRE1 or bZIP60 led to N. attenuate plants more susceptible to A. alternata, which were associated with reduced gene expressions of Feruloyl-CoA 6'-hydroxylase 1 (F6'H1), a gene encoding a key enzyme for phytoalexin scopoletin and scopolin biosynthesis. Further, electromobility shift assays (EMSA) indicated that bZIP60 protein of spliced form could directly bind to the promoter region of F6'H1 in vitro. JA signaling pathway is required for N. attenuate resistance to A. alternata. Interestingly, the fungus-elicited transcriptional levels of BiP, PDI, CNX 1 -like, CRT, IRE1, and bZIP60(s) were all significantly decreased in JA-deficient or JA-insensitive plants. Meanwhile, those genes were significantly induced by methyl jasmonate (MeJA) when applied exogenously. However, the transcriptional levels of JA-regulated genes allene oxide synthase (AOS) and lipoxygenease 3 (LOX3) were not affected in plants impaired with IRE1-bZIP60 pathway. Thus, it is concluded that IRE1-bZIP60 pathway is required for N. attenuate resistance to A. alternate, and JA signaling pathway plays an important role in the elicitation of chaperone protein genes and IRE1-bZIP60 pathway.
关键词	unfolded protein response inositol-requiring enzyme 1 (IRE1) bZIP60 Feruloyl-CoA 6 '-hydroxylase 1 (F6 ' H1) jasmonate (JA)
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/65162
专题	资源植物与生物技术所级重点实验室
通讯作者	Wu, Jinsong
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Kunming, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xu, Zhen,Song, Na,Ma, Lan,et al. IRE1-bZIP60 Pathway Is Required for Nicotiana attenuata Resistance to Fungal Pathogen Alternaria alternata[J]. FRONTIERS IN PLANT SCIENCE,2019,10:10.
APA	Xu, Zhen,Song, Na,Ma, Lan,&Wu, Jinsong.(2019).IRE1-bZIP60 Pathway Is Required for Nicotiana attenuata Resistance to Fungal Pathogen Alternaria alternata.FRONTIERS IN PLANT SCIENCE,10,10.
MLA	Xu, Zhen,et al."IRE1-bZIP60 Pathway Is Required for Nicotiana attenuata Resistance to Fungal Pathogen Alternaria alternata".FRONTIERS IN PLANT SCIENCE 10(2019):10.

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