Novel dual-color drug screening model for GLUT4 translocation in adipocytes

	Novel dual-color drug screening model for GLUT4 translocation in adipocytes
	Lu, Yanting 1,3; Ma, Xiuli 1,3; Kong, Qinghua 1; Xu, Yuhui 1; Hu, Jing 1,3; Wang, Fang 1,3; Qin, Wanying 1,3; Wang, Libin 2; Xiong, Wenyong1,2
	2019-02-01
发表期刊	MOLECULAR AND CELLULAR PROBES
ISSN	0890-8508
卷号	43 页码:6-12
摘要	Insulin-responsive glucose transporter type 4 (GLUT4) translocation plays a major role in controlling glucose uptake in adipose tissue and muscle, maintaining homeostasis and preventing hyperglycemia. Screening for chemicals enhancing GLUT4 translocation is an approach for identifying hits of drug development for type 2 diabetes. Here we developed a novel functional dual-color probe, pHluorin-GLUT4-mOrange2, and constructed 3T3-L1 adipocytes based screening system to simply and efficiently screen new compounds stimulating GLUT4 translocation. Based on this system, we successfully identified a few hits facilitating GLUT4 translocation. In conclusion, we developed an easy-to-apply dual color GLUT4 probe to monitor GLUT4 translocation in insulin-responsive cells, which could be alternatively employed to high-throughput screen compounds regulating GLUT4 translocation and glucose uptake, even to dissect GLTU4 approaching, docking and fusion with the plasma membrane (PM), and to reveal relevant molecular mechanisms involved in these steps as expected.
关键词	GLUT4 pHluorin Screening Glucose uptake
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64245
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Wang, Libin; Xiong, Wenyong
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Ningxia Med Univ, Dept Beijing Natl Biochip Res Ctr Subctr Ningxia, Gen Hosp, Yinchuan 750004, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lu, Yanting,Ma, Xiuli,Kong, Qinghua,et al. Novel dual-color drug screening model for GLUT4 translocation in adipocytes[J]. MOLECULAR AND CELLULAR PROBES,2019,43:6-12.
APA	Lu, Yanting.,Ma, Xiuli.,Kong, Qinghua.,Xu, Yuhui.,Hu, Jing.,...&Xiong, Wenyong.(2019).Novel dual-color drug screening model for GLUT4 translocation in adipocytes.MOLECULAR AND CELLULAR PROBES,43,6-12.
MLA	Lu, Yanting,et al."Novel dual-color drug screening model for GLUT4 translocation in adipocytes".MOLECULAR AND CELLULAR PROBES 43(2019):6-12.

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