Novel dual-color drug screening model for GLUT4 translocation in adipocytes
Lu, Yanting1,3; Ma, Xiuli1,3; Kong, Qinghua1; Xu, Yuhui1; Hu, Jing1,3; Wang, Fang1,3; Qin, Wanying1,3; Wang, Libin2; Xiong, Wenyong1,2
AbstractInsulin-responsive glucose transporter type 4 (GLUT4) translocation plays a major role in controlling glucose uptake in adipose tissue and muscle, maintaining homeostasis and preventing hyperglycemia. Screening for chemicals enhancing GLUT4 translocation is an approach for identifying hits of drug development for type 2 diabetes. Here we developed a novel functional dual-color probe, pHluorin-GLUT4-mOrange2, and constructed 3T3-L1 adipocytes based screening system to simply and efficiently screen new compounds stimulating GLUT4 translocation. Based on this system, we successfully identified a few hits facilitating GLUT4 translocation. In conclusion, we developed an easy-to-apply dual color GLUT4 probe to monitor GLUT4 translocation in insulin-responsive cells, which could be alternatively employed to high-throughput screen compounds regulating GLUT4 translocation and glucose uptake, even to dissect GLTU4 approaching, docking and fusion with the plasma membrane (PM), and to reveal relevant molecular mechanisms involved in these steps as expected.
KeywordGLUT4 pHluorin Screening Glucose uptake
Indexed BySCI
Document Type期刊论文
Corresponding AuthorWang, Libin; Xiong, Wenyong
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Ningxia Med Univ, Dept Beijing Natl Biochip Res Ctr Subctr Ningxia, Gen Hosp, Yinchuan 750004, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lu, Yanting,Ma, Xiuli,Kong, Qinghua,et al. Novel dual-color drug screening model for GLUT4 translocation in adipocytes[J]. MOLECULAR AND CELLULAR PROBES,2019,43:6-12.
APA Lu, Yanting.,Ma, Xiuli.,Kong, Qinghua.,Xu, Yuhui.,Hu, Jing.,...&Xiong, Wenyong.(2019).Novel dual-color drug screening model for GLUT4 translocation in adipocytes.MOLECULAR AND CELLULAR PROBES,43,6-12.
MLA Lu, Yanting,et al."Novel dual-color drug screening model for GLUT4 translocation in adipocytes".MOLECULAR AND CELLULAR PROBES 43(2019):6-12.
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