Structural analysis and anticoagulant activities of two sulfated polysaccharides from the sea cucumber Holothuria coluber

doi:10.1016/j.ijbiomac.2018.04.175

KIB OpenIR

	Structural analysis and anticoagulant activities of two sulfated polysaccharides from the sea cucumber Holothuria coluber
	Yang, Wenjiao; Cai, Ying; Yin, Ronghua; Lin, Lisha; Li, Zhongkun; Wu, Mingyi; Zhao, Jinhua
通讯作者	Li, Zhongkun(yayylzk@163.com) ; Wu, Mingyi(wumingyi@mail.kib.ac.cn) ; Zhao, Jinhua(zhaojinhua@mail.kib.ac.cn)
	2018
发表期刊	PHYTOCHEMISTRY LETTERS
ISSN	1874-3900
卷号	26 页码:50-54
摘要	Sulfated polysaccharides such as fucosylated glycosaminoglycan and fucan sulfate from echinoderm possess complex chemical structure and various biological activities. The two sulfated polysaccharides were purified from the low-value sea cucumber Holothuria coluber. Their physicochemical properties and chemical structures were analyzed and characterized by chemical and instrumental methods. Structural analysis clarified that the sea cucumber fucosylated glycosaminoglycan contains a chondroitin sulfate-like backbone and fucosyl branches with four various sulfation patterns. The fucan sulfate with molecular weight of 64.6 kDa comprises a central core of regular alpha(1 -> 4)-linked tetrasaccharide repeating units, each of which is linked by a 4-O-sulfated fucose residue. Anticoagulant assays indicated that these sulfated polysaccharides possessed strong APTT prolonging activities and intrinsic factor Xase inhibitory activities, both of which decreased with the reduction of their molecular weights. Our results expand knowledge on the structural types of sulfated polysaccharides from sea cucumbers and further illustrate their functionality. (C) 2018 Elsevier B.V. All rights reserved.
关键词	Fucosylated glycosaminoglycan Fucan sulfate Polysaccharide Anticoagulant Sea cucumber
DOI	10.1016/j.ijbiomac.2018.04.175
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000438662500118
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/63320
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yang, Wenjiao,Cai, Ying,Yin, Ronghua,et al. Structural analysis and anticoagulant activities of two sulfated polysaccharides from the sea cucumber Holothuria coluber[J]. PHYTOCHEMISTRY LETTERS,2018,26:50-54.
APA	Yang, Wenjiao.,Cai, Ying.,Yin, Ronghua.,Lin, Lisha.,Li, Zhongkun.,...&Zhao, Jinhua.(2018).Structural analysis and anticoagulant activities of two sulfated polysaccharides from the sea cucumber Holothuria coluber.PHYTOCHEMISTRY LETTERS,26,50-54.
MLA	Yang, Wenjiao,et al."Structural analysis and anticoagulant activities of two sulfated polysaccharides from the sea cucumber Holothuria coluber".PHYTOCHEMISTRY LETTERS 26(2018):50-54.