Metabolomic analysis of cholestatic liver damage in mice

doi:10.1016/j.fct.2018.07.022

	Metabolomic analysis of cholestatic liver damage in mice
	Yang, Rui 1,2; Zhao, Qi1,2 ; Hu, Dan-Dan 1,3,4; Xiao, Xue-Rong 1; Huang, Jian-Feng 1,2; Li, Fei 1
	2018-10-01
发表期刊	FOOD AND CHEMICAL TOXICOLOGY
ISSN	0278-6915
卷号	120 页码:253-260
摘要	Cholestasis is characterized by the obstruction of bile duct, including primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC). The complicated etiology and injury mechanism greatly limits the development of new drugs for its treatment. To better understand the mechanism of cholestatic liver damage, ultra-performance liquid chromatography-linked electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) and multivariate data analysis were used to determine the metabolic changes in three recognized mouse cholestasis models. The cholestatic liver damage was generated by alphanaphthyl isothiocyanate (ANIT), 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) and lithocholic acid (LCA). The results indicated that the levels of bile acids were commonly increased in plasma of three mouse cholestasis models, while arginine was decreased. The level of plasma glutathione was decreased in ANIT- and LCA-induced intrahepatic PBC and PSC, respectively. But, the liver glutathione was decreased in DDC induced extrahepatic PSC. The level of plasma phospholipids was elevated in ANIT and DDC models, whereas that was depleted in LCA model. And protoporphyrin IX was significantly increased in the liver of DDC model. These metabolomics data could potentially distinguish the metabolic differences of three types of cholestasis, contributing to the understanding of the potential mechanism of cholestatic liver damage.
关键词	Cholestatic liver damage PBC PSC Metabolomics QTOFMS
DOI	10.1016/j.fct.2018.07.022
语种	英语
WOS记录号	WOS:000447482200026
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/62793
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Li, Fei
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China 4.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yang, Rui,Zhao, Qi,Hu, Dan-Dan,et al. Metabolomic analysis of cholestatic liver damage in mice[J]. FOOD AND CHEMICAL TOXICOLOGY,2018,120:253-260.
APA	Yang, Rui,Zhao, Qi,Hu, Dan-Dan,Xiao, Xue-Rong,Huang, Jian-Feng,&Li, Fei.(2018).Metabolomic analysis of cholestatic liver damage in mice.FOOD AND CHEMICAL TOXICOLOGY,120,253-260.
MLA	Yang, Rui,et al."Metabolomic analysis of cholestatic liver damage in mice".FOOD AND CHEMICAL TOXICOLOGY 120(2018):253-260.