Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro
Wu, Xin1,2; Xie, Chuan-qi1,2; Zhu, Qiang-qiang1,2; Wang, Ming-yue1,2; Sun, Bin1,2; Huang, Yan-ping1,2; Shen, Chang1,2; An, Meng-Fei1,2; Zhao, Yun-li1,2,3; Wang, Xuan-jun1,4,5; Sheng, Jun1,4,5
2018-10-08
发表期刊FOOD & NUTRITION RESEARCH
ISSN1654-6628
卷号62页码:11
摘要Background: Green tea (Camelliasinensis [L.] Kuntze) belongs to the plant family Theaceae and is mainly distributed in East Asia, the Indian subcontinent and Southeast Asia. This plant has been proven to be beneficial to human health, and green tea is the second most consumed beverage in the world after water. However, until now, the effect of green tea aqueous extract (GTE) upon postmenopausal osteoporosis has remained unclear. In this study, we investigated the therapeutic effects of GTE on estrogen deficiency-induced osteoporosis and explored the possible mechanisms in vivo and in vitro. Materials and methods: Ovariectomized (OVX) female rats were orally administered with GTE at doses of 60, 120, and 370 mg kg(-1) for 13 consecutive weeks. The biochemical parameters, bone gla protein, alkaline phosphatase, acid phosphatase, estrogen, interleukin-1 beta, and interleukin-6 in blood samples were detected, and histological change in bones was analyzed by hematoxylin and eosin staining. Meanwhile, the mechanisms of GTE on osteoclast formation were explored in RAW 264.7 cells induced by receptor activation of the nuclear factor kappa B ligand (RANKL). Results: The results showed that GTE could increase bone mass and inhibit trabecular bone loss in OVX rats. Furthermore, real-time quantitative reverse transcription polymerase chain reaction analysis from in vitro experiments also showed that GTE reduced the mRNA expression of osteoclast-associated genes such as cathepsin K (cath-K), c-Fos, matrix metalloproteinase 9, nuclear factor of activated T cells cytoplasmic 1 (NFATcl) and tartrate-resistant acid phosphatase. In addition, GTE caused a reduction in the protein levels of NFATcl, c-Fos, c-src and cath-K. Conclusion: Evidence from both animal models and in vitro experiments suggested that GTE might effectively ameliorate the symptoms of osteoporosis in OVX rats and inhibit RANKL-induced osteoclast-specific gene and protein expression.
关键词green tea aqueous extract osteoporosis ovariectomy receptor activator of the nuclear factor kappa B ligand osteoclast
资助信息Scholarship for Talent Academic Leader of Yunnan Province; Scholarship for Young Academic and Technological Leaders of Yunnan Province; Science and Technology Major Project of Yunnan Province
语种英语
资助项目Scholarship for Talent Academic Leader of Yunnan Province[2017HA015] ; Scholarship for Young Academic and Technological Leaders of Yunnan Province[2015HB037] ; Science and Technology Major Project of Yunnan Province[2017ZF003]
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/62775
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Zhao, Yun-li; Wang, Xuan-jun; Sheng, Jun
作者单位1.Yunnan Agr Univ, Key Lab Pu Erh Tea Sci, Minist Educ, Kunming, Yunnan, Peoples R China
2.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China
4.Yunnan Agr Univ, Coll Sci, Kunming, Yunnan, Peoples R China
5.State Key Lab Conservat & Utilizat Bioresources Y, Kunming, Yunnan, Peoples R China
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GB/T 7714
Wu, Xin,Xie, Chuan-qi,Zhu, Qiang-qiang,et al. Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro[J]. FOOD & NUTRITION RESEARCH,2018,62:11.
APA Wu, Xin.,Xie, Chuan-qi.,Zhu, Qiang-qiang.,Wang, Ming-yue.,Sun, Bin.,...&Sheng, Jun.(2018).Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro.FOOD & NUTRITION RESEARCH,62,11.
MLA Wu, Xin,et al."Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro".FOOD & NUTRITION RESEARCH 62(2018):11.
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