Glycoside Hydrolase Family 39 beta-Xylosidase of Sphingomonas Showing Salt/Ethanol/Trypsin Tolerance, Low-pH/Low-Temperature Activity, and Transxylosylation Activity
Li, Na1,2,3; Han, Xiaowei1,2,3; Xu, Shujing1,2,3; Li, Chunyan1,2,3; Wei, Xin4; Liu, Yu1,2,3; Zhang, Rui1,2,3; Tang, Xianghua1,2,3; Zhou, Junpei1,2,3; Huang, Zunxi1,2,3
2018-09-12
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
卷号66期号:36页码:9465-9472
摘要Mining for novel enzymes from new microorganisms is a way to obtain beta-xylosidases with promising applications. A Sphingomonas beta-xylosidase was expressed in Escherichia coli. The purified recombinant enzyme (rJB13GH39) was most active at pH 4.5 and 50 degrees C, retaining 10%-50% of its maximum activity at 0-20 degrees C. Most salts and chemical reagents including 3.0%-20.0% (w/v) NaCl showed little or no effect on the enzymatic activity. rJB13GH39 exhibited 71.9% and 55.2% activity in 10.0% and 15.0% (v/v) ethanol, respectively. rJB13GH39 was stable below 60 degrees C in 3.0%-30.0% (w/v) NaCl, 3.0%-20.0% (v/v) ethanol, and 2.2-87.0 mg/mL trypsin. The enzyme transferred one xylosyl moiety to certain sugars and alcohols. The salt/ethanol tolerance and low-temperature activity of the enzyme may be attributed to its high structural flexibility caused by high proportions of small amino acids ACDGNSTV and random coils.
关键词beta-xylosidase salt tolerance ethanol tolerance trypsin tolerance transxylosylation low-temperature activity glycoside hydrolase family 39 Sphingomonas sp
资助信息National Key Research and Development Program of China; National Natural Science Foundation of China; Yunling Scholars; Yunling Industry Leading Talents; Reserve Talents Project for Young and Middle-Aged Academic and Technical Leaders of Yunnan Province; National Innovation and Entrepreneurship Training Program for College Students of China
语种英语
资助项目National Key Research and Development Program of China[2017YFB0308401] ; National Natural Science Foundation of China[31660445] ; Yunling Scholars[2015 56] ; Yunling Industry Leading Talents[2014 1782] ; Reserve Talents Project for Young and Middle-Aged Academic and Technical Leaders of Yunnan Province[2015HB033] ; National Innovation and Entrepreneurship Training Program for College Students of China[201710681016] ; National Innovation and Entrepreneurship Training Program for College Students of China[201810681017]
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/62426
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Zhou, Junpei; Huang, Zunxi
作者单位1.Yunnan Normal Univ, Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Minist Educ, Kunming 650500, Yunnan, Peoples R China
2.Yunnan Normal Univ, Coll Life Sci, Kunming 650500, Yunnan, Peoples R China
3.Key Lab Yunnan Biomass Energy & Biotechnol Enviro, Kunming 650500, Yunnan, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
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GB/T 7714
Li, Na,Han, Xiaowei,Xu, Shujing,et al. Glycoside Hydrolase Family 39 beta-Xylosidase of Sphingomonas Showing Salt/Ethanol/Trypsin Tolerance, Low-pH/Low-Temperature Activity, and Transxylosylation Activity[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018,66(36):9465-9472.
APA Li, Na.,Han, Xiaowei.,Xu, Shujing.,Li, Chunyan.,Wei, Xin.,...&Huang, Zunxi.(2018).Glycoside Hydrolase Family 39 beta-Xylosidase of Sphingomonas Showing Salt/Ethanol/Trypsin Tolerance, Low-pH/Low-Temperature Activity, and Transxylosylation Activity.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,66(36),9465-9472.
MLA Li, Na,et al."Glycoside Hydrolase Family 39 beta-Xylosidase of Sphingomonas Showing Salt/Ethanol/Trypsin Tolerance, Low-pH/Low-Temperature Activity, and Transxylosylation Activity".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 66.36(2018):9465-9472.
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