Backbone and side-chain chemical shift assignments of MarH, a critical intermediary epimerase for biosynthesis of Maremycins in Streptomyces
Liu, Bin1,2; Fang, Shiqi1,2; Ma, Xiaofang1,2; Bai, Zhiqiang1,2; Hu, Kaifeng1
2018-10-01
发表期刊BIOMOLECULAR NMR ASSIGNMENTS
ISSN1874-2718
卷号12期号:2页码:335-338
摘要MarH is an essential epimerase that catalyzes the isomerization of 3R--methyl-indolepyruvate (-MeInPy) to 3S--MeInPy, which is the important precursor for biosynthesis of Maremycins. Biophysical study of the structure of MarH would be informative for better understanding of its catalytic mechanism and feasible application of the enzyme in isomerization reaction. Here, we report the backbone and side-chain NMR chemical shift assignments of MarH, which lays a foundation for further structural and mechanical study of the enzyme.
关键词MarH Epimerase NMR Chemical shift assignments
资助信息National Key Research and Development Program of China; National Natural Science Foundation of China; Yunnan Provincial Science and Technology Department
DOI10.1007/s12104-018-9835-2
语种英语
资助项目National Key Research and Development Program of China[2017YFC0906900] ; National Natural Science Foundation of China[21573258] ; Yunnan Provincial Science and Technology Department[2015FA028]
WOS研究方向Biophysics ; Spectroscopy
WOS类目Biophysics ; Spectroscopy
WOS记录号WOS:000443824500022
出版者SPRINGER
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/62303
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Hu, Kaifeng
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Liu, Bin,Fang, Shiqi,Ma, Xiaofang,et al. Backbone and side-chain chemical shift assignments of MarH, a critical intermediary epimerase for biosynthesis of Maremycins in Streptomyces[J]. BIOMOLECULAR NMR ASSIGNMENTS,2018,12(2):335-338.
APA Liu, Bin,Fang, Shiqi,Ma, Xiaofang,Bai, Zhiqiang,&Hu, Kaifeng.(2018).Backbone and side-chain chemical shift assignments of MarH, a critical intermediary epimerase for biosynthesis of Maremycins in Streptomyces.BIOMOLECULAR NMR ASSIGNMENTS,12(2),335-338.
MLA Liu, Bin,et al."Backbone and side-chain chemical shift assignments of MarH, a critical intermediary epimerase for biosynthesis of Maremycins in Streptomyces".BIOMOLECULAR NMR ASSIGNMENTS 12.2(2018):335-338.
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