An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose

doi:10.1007/s00216-018-1276-3

	An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose
	Huang, Tao 1,2; Yu, Lingling 1,2; Ma, Xiaofang 1,2; Hu, Kaifeng1
	2018-10-01
发表期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
卷号	410 期号:26 页码:6705-6711
摘要	A wide variety of methods, such as enzymatic methods, LC-MS, and LC-MS/MS, are currently available for the concentration determination of plasma glucose in studies of diabetes, obesity, exercise, etc. However, these methods rarely discriminate endogenous and exogenous glucose in plasma. A novel NMR strategy for discriminative quantification of the endogenous and exogenous glucose in plasma has been developed using an adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) with uniformly C-13-labeled glucose as a tracer of exogenous glucose. This method takes advantage of the distinct H-1-C-13 chemical shifts of the hemiacetal group of the -D-glucopyranose and makes use of the C-13-C-13 one-bond J-coupling ((1)J(CC)) in uniformly C-13-labeled glucose to differentiate the H-1-C-13 HSQC signal of labeled glucose from that of its natural counterpart when data are acquired in high-resolution mode. The molar ratio between the endogenous and exogenous plasma glucose can then be calculated from the peak intensities of the natural and labeled glucose. The accuracy and precision of the method were evaluated using a series of standard mixtures of natural and uniformly C-13-labeled glucose with varied but known concentrations. Application of this method is demonstrated for the quantification of endogenous and exogenous glucose in plasma derived from healthy and diabetic cynomolgus monkeys. The results nicely agree with our previous LC-MS/MS results. Considering the natural abundance of C-13 isotope at the level of 1.1% in endogenous glucose, comparable peak intensities of quantitatively measurable signals derived from natural and labeled glucose imply that the ID-HSQC can tolerate a significantly high ratio of isotope dilution, with labeled/natural glucose at 1%. We expect that the ID-HSQC method can serve as an alternative approach to the biomedical or clinical studies of glucose metabolism.
关键词	Isotope dilution HSQC Carbon-13 labeling Quantification Glucose
DOI	10.1007/s00216-018-1276-3
语种	英语
WOS记录号	WOS:000444236400002
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/62302
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Hu, Kaifeng
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Huang, Tao,Yu, Lingling,Ma, Xiaofang,et al. An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(26):6705-6711.
APA	Huang, Tao,Yu, Lingling,Ma, Xiaofang,&Hu, Kaifeng.(2018).An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(26),6705-6711.
MLA	Huang, Tao,et al."An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.26(2018):6705-6711.