| An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose | |
Huang, Tao1,2; Yu, Lingling1,2; Ma, Xiaofang1,2; Hu, Kaifeng1
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| 2018-10-01 | |
| Source Publication | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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| ISSN | 1618-2642 |
| Volume | 410Issue:26Pages:6705-6711 |
| Abstract | A wide variety of methods, such as enzymatic methods, LC-MS, and LC-MS/MS, are currently available for the concentration determination of plasma glucose in studies of diabetes, obesity, exercise, etc. However, these methods rarely discriminate endogenous and exogenous glucose in plasma. A novel NMR strategy for discriminative quantification of the endogenous and exogenous glucose in plasma has been developed using an adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) with uniformly C-13-labeled glucose as a tracer of exogenous glucose. This method takes advantage of the distinct H-1-C-13 chemical shifts of the hemiacetal group of the -D-glucopyranose and makes use of the C-13-C-13 one-bond J-coupling ((1)J(CC)) in uniformly C-13-labeled glucose to differentiate the H-1-C-13 HSQC signal of labeled glucose from that of its natural counterpart when data are acquired in high-resolution mode. The molar ratio between the endogenous and exogenous plasma glucose can then be calculated from the peak intensities of the natural and labeled glucose. The accuracy and precision of the method were evaluated using a series of standard mixtures of natural and uniformly C-13-labeled glucose with varied but known concentrations. Application of this method is demonstrated for the quantification of endogenous and exogenous glucose in plasma derived from healthy and diabetic cynomolgus monkeys. The results nicely agree with our previous LC-MS/MS results. Considering the natural abundance of C-13 isotope at the level of 1.1% in endogenous glucose, comparable peak intensities of quantitatively measurable signals derived from natural and labeled glucose imply that the ID-HSQC can tolerate a significantly high ratio of isotope dilution, with labeled/natural glucose at 1%. We expect that the ID-HSQC method can serve as an alternative approach to the biomedical or clinical studies of glucose metabolism. |
| Keyword | Isotope dilution HSQC Carbon-13 labeling Quantification Glucose |
| DOI | 10.1007/s00216-018-1276-3 |
| Language | 英语 |
| WOS ID | WOS:000444236400002 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/62302 |
| Collection | 植物化学与西部植物资源持续利用国家重点实验室 |
| Corresponding Author | Hu, Kaifeng |
| Affiliation | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Huang, Tao,Yu, Lingling,Ma, Xiaofang,et al. An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(26):6705-6711. |
| APA | Huang, Tao,Yu, Lingling,Ma, Xiaofang,&Hu, Kaifeng.(2018).An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(26),6705-6711. |
| MLA | Huang, Tao,et al."An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.26(2018):6705-6711. |
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