An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose
Huang, Tao1,2; Yu, Lingling1,2; Ma, Xiaofang1,2; Hu, Kaifeng1
2018-10-01
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume410Issue:26Pages:6705-6711
AbstractA wide variety of methods, such as enzymatic methods, LC-MS, and LC-MS/MS, are currently available for the concentration determination of plasma glucose in studies of diabetes, obesity, exercise, etc. However, these methods rarely discriminate endogenous and exogenous glucose in plasma. A novel NMR strategy for discriminative quantification of the endogenous and exogenous glucose in plasma has been developed using an adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) with uniformly C-13-labeled glucose as a tracer of exogenous glucose. This method takes advantage of the distinct H-1-C-13 chemical shifts of the hemiacetal group of the -D-glucopyranose and makes use of the C-13-C-13 one-bond J-coupling ((1)J(CC)) in uniformly C-13-labeled glucose to differentiate the H-1-C-13 HSQC signal of labeled glucose from that of its natural counterpart when data are acquired in high-resolution mode. The molar ratio between the endogenous and exogenous plasma glucose can then be calculated from the peak intensities of the natural and labeled glucose. The accuracy and precision of the method were evaluated using a series of standard mixtures of natural and uniformly C-13-labeled glucose with varied but known concentrations. Application of this method is demonstrated for the quantification of endogenous and exogenous glucose in plasma derived from healthy and diabetic cynomolgus monkeys. The results nicely agree with our previous LC-MS/MS results. Considering the natural abundance of C-13 isotope at the level of 1.1% in endogenous glucose, comparable peak intensities of quantitatively measurable signals derived from natural and labeled glucose imply that the ID-HSQC can tolerate a significantly high ratio of isotope dilution, with labeled/natural glucose at 1%. We expect that the ID-HSQC method can serve as an alternative approach to the biomedical or clinical studies of glucose metabolism.
KeywordIsotope dilution HSQC Carbon-13 labeling Quantification Glucose
DOI10.1007/s00216-018-1276-3
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000444236400002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/62302
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorHu, Kaifeng
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Huang, Tao,Yu, Lingling,Ma, Xiaofang,et al. An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(26):6705-6711.
APA Huang, Tao,Yu, Lingling,Ma, Xiaofang,&Hu, Kaifeng.(2018).An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(26),6705-6711.
MLA Huang, Tao,et al."An adapted isotope dilution H-1-C-13 heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.26(2018):6705-6711.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Tao]'s Articles
[Yu, Lingling]'s Articles
[Ma, Xiaofang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Tao]'s Articles
[Yu, Lingling]'s Articles
[Ma, Xiaofang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Tao]'s Articles
[Yu, Lingling]'s Articles
[Ma, Xiaofang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.