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Single glucose molecule transport process revealed by force tracing and molecular dynamics simulations
Pan, Yangang1; Zhang, Yuebin3; Gongpan, Pianchou4,8; Zhang, Qingrong1,5; Huang, Siteng3; Wang, Bin6,7; Xu, Bingqian6,7; Shan, Yuping5; Xiong, Wenyong4; Li, Guohui3; Wang, Hongda1,2,8
2018-09-01
发表期刊NANOSCALE HORIZONS
ISSN2055-6756
卷号3期号:5页码:9
摘要Transporting individual molecules across cell membranes is a fundamental process in cellular metabolism. Although the crystal diffraction technique has greatly contributed to our understanding of the structures of the involved transporters, a description of the dynamic transport mechanism at the single-molecule level has been extremely elusive. In this study, we applied atomic force microscopy (AFM)-based force tracing to directly monitor the transport of a single molecule, D-glucose, across living cell membranes. Our results show that the force to transport a single molecule of D-glucose across cell membranes is 37 +/- 9 pN, and the corresponding transport interval is approximately 20 ms, while the average speed is approximately 0.3 mu m s(-1). Furthermore, our calculated force profile from molecular dynamics simulations showed quantitatively good agreement with the force tracing observation and revealed detailed information regarding the glucose transport path, indicating that two salt bridges, K38/E299 and K300/E426, play critical roles during glucose transport across glucose transporter 1 (GLUT1). This role was further verified using biological experiments that disrupted these two bridges and measured the uptake of glucose into the cells. Our approaches led to the first unambiguous description of the glucose transport process across cell membranes at the single-molecule level and demonstrated the biological importance of the two salt bridges for transporting glucose across GLUT1.
资助信息National Key R&D Program of China; National Natural Science Foundation of China; Science and Technology Department of Yunnan Province; Jilin Provincial Science Research Foundation of China
语种英语
资助项目National Key R&D Program of China[2017YFA0505300] ; National Natural Science Foundation of China[21727816] ; National Natural Science Foundation of China[21525314] ; National Natural Science Foundation of China[21721003] ; National Natural Science Foundation of China[21573217] ; National Natural Science Foundation of China[31330082] ; National Natural Science Foundation of China[21673023] ; Science and Technology Department of Yunnan Province[2017FA044] ; Science and Technology Department of Yunnan Province[2013HA023] ; Jilin Provincial Science Research Foundation of China[20160520133JH]
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/62046
专题中国科学院昆明植物研究所
通讯作者Shan, Yuping; Xiong, Wenyong; Li, Guohui; Wang, Hongda
作者单位1.Chinese Acad Sci, State Key Lab Electroanalyt Chem, Res Ctr Biomembran, Changchun Inst Appl Chem, Jilin 130022, Jilin, Peoples R China
2.Qing Dao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, State Key Lab Mol React Dynam, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
5.Adv Inst Mat Sci, Sch Chem & Life Sci, Changchun 130012, Jilin, Peoples R China
6.Univ Georgia, Single Mol Study Lab, Coll Engn, Athens, GA 30602 USA
7.Univ Georgia, Nanoscale Sci & Engn Ctr, Athens, GA 30602 USA
8.Chinese Acad Sci, Grad Univ, Beijing 100019, Peoples R China
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GB/T 7714
Pan, Yangang,Zhang, Yuebin,Gongpan, Pianchou,et al. Single glucose molecule transport process revealed by force tracing and molecular dynamics simulations[J]. NANOSCALE HORIZONS,2018,3(5):9.
APA Pan, Yangang.,Zhang, Yuebin.,Gongpan, Pianchou.,Zhang, Qingrong.,Huang, Siteng.,...&Wang, Hongda.(2018).Single glucose molecule transport process revealed by force tracing and molecular dynamics simulations.NANOSCALE HORIZONS,3(5),9.
MLA Pan, Yangang,et al."Single glucose molecule transport process revealed by force tracing and molecular dynamics simulations".NANOSCALE HORIZONS 3.5(2018):9.
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