Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways

	Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways
	Zeng Qiang 1,2; Ko, Chun-Hay 1,2,3; Siu, Wing-Sum 1,2; Li Kai-Kai 1,2; Wong, Chun-Wai 1,2; Han Xiao-Qiang 1,2,3; Yang Liu 4; Lau, Clara Bik-San 1,2,3; Hu Jiang-Miao4 ; Leung, Ping-Chung 1,2,3
	2018-07-01
发表期刊	CHINESE JOURNAL OF NATURAL MEDICINES
ISSN	2095-6975
卷号	16 期号:7 页码:481-489
摘要	Dendrobii Caulis (DC), named 'Shihu' in Chinese, is a precious herb in traditional Chinese medicine. It is widely used to nourish stomach, enhance body fluid production, tonify "Yin" and reduce heat. More than thirty Dendrobium species are used as folk medicine. Some compounds from DC exhibit inhibitory effects on macrophage inflammation. In the present study, we compared the anti-inflammatory effects among eight Dendrobium species. The results provided evidences to support Dendrobium as folk medicine, which exerted its medicinal function partially by its inhibitory effects on inflammation. To investigate the anti-inflammatory effect of Dendrobium species, mouse macrophage cell line RAW264.7 was activated by lipopolysaccharide. The nitric oxide (NO) level was measured using Griess reagent while the pro-inflammatory cytokines were tested by ELISA. The protein expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and mitogen-activated protein kinases (MAPKs) phosphorylation were evaluated by Western blotting analysis. Among the eight Dendrobium species, both water extracts of D. thyrsiflorum B.S. Williams (DTW) and D. chrysotoxum Lindl (DCHW) showed most significant inhibitory effects on NO production in a concentration-dependent manner. DTW also significantly reduced TNF-alpha, MCP-1, and IL-6 production. Further investigations showed that DTW suppressed iNOS and COX-2 expression as well as ERK and JNK phosphorylation, suggesting that the inhibitory effects of DTW on LPS-induced macrophage inflammation was through the suppression of MAPK pathways. In conclusion, D. thyrsiflorum B.S. Williams was demonstrated to have potential to be used as alternative or adjuvant therapy for inflammation.
关键词	Dendrobium Species Anti-inflammation Macrophage Mapk Pathways
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/61681
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Univ Hong Kong, Inst Chinese Med, Hong Kong, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Hong Kong, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zeng Qiang,Ko, Chun-Hay,Siu, Wing-Sum,et al. Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2018,16(7):481-489.
APA	Zeng Qiang.,Ko, Chun-Hay.,Siu, Wing-Sum.,Li Kai-Kai.,Wong, Chun-Wai.,...&Leung, Ping-Chung.(2018).Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways.CHINESE JOURNAL OF NATURAL MEDICINES,16(7),481-489.
MLA	Zeng Qiang,et al."Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways".CHINESE JOURNAL OF NATURAL MEDICINES 16.7(2018):481-489.