Photoinhibition of photosystem I in Nephrolepis falciformis depends on reactive oxygen species generated in the chloroplast stroma

doi:10.1007/s11120-018-0484-1

	Photoinhibition of photosystem I in Nephrolepis falciformis depends on reactive oxygen species generated in the chloroplast stroma
	Huang, Wei1 ; Tikkanen, Mikko 2; Zhang, Shi-Bao1
	2018-07-01
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	137 期号:1 页码:129-140
摘要	We studied how high light causes photoinhibition of photosystem I (PSI) in the shade-demanding fern Nephrolepis falciformis, in an attempt to understand the mechanism of PSI photoinhibition under natural field conditions. Intact leaves were treated with constant high light and fluctuating light. Detached leaves were treated with constant high light in the presence and absence of methyl viologen (MV). Chlorophyll fluorescence and P700 signal were determined to estimate photoinhibition. PSI was highly oxidized under high light before treatments. N. falciformis showed significantly stronger photoinhibition of PSI and PSII under constant high light than fluctuating light. These results suggest that high levels of P700 oxidation ratio cannot prevent PSI photoinhibition under high light in N. falciformis. Furthermore, photoinhibition of PSI in N. falciformis was largely accelerated in the presence of MV that promotes the production of superoxide anion radicals in the chloroplast stroma by accepting electrons from PSI. From these results, we propose that photoinhibition of PSI in N. falciformis is mainly caused by superoxide radicals generated in the chloroplast stroma, which is different from the mechanism of PSI photoinhibition in Arabidopsis thaliana and spinach. Here, we provide some new insights into the PSI photoinhibition under natural field conditions.
关键词	Chlorophyll Fluorescence High Light Methyl Viologen Photoinhibition Photosystem Superoxide
DOI	10.1007/s11120-018-0484-1
语种	英语
WOS记录号	WOS:000435365700010
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/61426
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Univ Turku, Dept Biochem, Mol Plant Biol, Turku 20014, Finland
推荐引用方式 GB/T 7714	Huang, Wei,Tikkanen, Mikko,Zhang, Shi-Bao. Photoinhibition of photosystem I in Nephrolepis falciformis depends on reactive oxygen species generated in the chloroplast stroma[J]. PHOTOSYNTHESIS RESEARCH,2018,137(1):129-140.
APA	Huang, Wei,Tikkanen, Mikko,&Zhang, Shi-Bao.(2018).Photoinhibition of photosystem I in Nephrolepis falciformis depends on reactive oxygen species generated in the chloroplast stroma.PHOTOSYNTHESIS RESEARCH,137(1),129-140.
MLA	Huang, Wei,et al."Photoinhibition of photosystem I in Nephrolepis falciformis depends on reactive oxygen species generated in the chloroplast stroma".PHOTOSYNTHESIS RESEARCH 137.1(2018):129-140.