Liquid chromatography-mass spectrometry combined with xanthine oxidase inhibition profiling for identifying the bioactive constituents from Cistanche deserticola

doi:10.1016/j.ijms.2018.03.009

	Liquid chromatography-mass spectrometry combined with xanthine oxidase inhibition profiling for identifying the bioactive constituents from Cistanche deserticola
	Chen, Xinglong 1,2; Deng, Zhentao 1,2; Huang, Xiaoyan 1,2; Geng, Changan 1,2; Chen, Jijun1,2
	2018-07-01
发表期刊	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
ISSN	1387-3806
卷号	430 页码:1-7
摘要	Xanthine oxidase (XO) inhibitor can block the synthesis of uric acid and is regarded as one of the therapeutic approaches of gout. The anti-XO constituents in the stem of Cistanche deserticola were characterized though LC-MS combined with xanthine oxidase inhibition profiling method. Seven phenylethanoid glycosides (1-7) and two phenylpropanoid glycosides (8-9) were identified from the bioactive extracts. Isoacteoside (1), kankanoside G (4) and cistanoside F (8) displayed crucial activity against XO with the IC50 values of 46.91, 85.31 mu M and 36.41 mu M respectively. The main mass fragmentation pathways of phenylethanoid glycosides were the losses of glucosyl, rhamanopyranosyl and phenylethoxyl groups in positive ion mode and the elimination of caffeoyl unit in negative ion mode. It shows that the stem of Cistanche deserticola is a functional food which performs potent anti-gout activity. (C) 2018 Elsevier B.V. All rights reserved.
关键词	Lc-ms Xanthine Oxidase Inhibitor Anti-gout Mass Fragmentation Pathway Cistanche Deserticola
DOI	10.1016/j.ijms.2018.03.009
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/61386
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xinglong,Deng, Zhentao,Huang, Xiaoyan,et al. Liquid chromatography-mass spectrometry combined with xanthine oxidase inhibition profiling for identifying the bioactive constituents from Cistanche deserticola[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2018,430:1-7.
APA	Chen, Xinglong,Deng, Zhentao,Huang, Xiaoyan,Geng, Changan,&Chen, Jijun.(2018).Liquid chromatography-mass spectrometry combined with xanthine oxidase inhibition profiling for identifying the bioactive constituents from Cistanche deserticola.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,430,1-7.
MLA	Chen, Xinglong,et al."Liquid chromatography-mass spectrometry combined with xanthine oxidase inhibition profiling for identifying the bioactive constituents from Cistanche deserticola".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 430(2018):1-7.