An anticoagulant fucan sulfate with hexasaccharide repeating units from the sea cucumber Holothuria albiventer | |
Cai, Ying1,2; Yang, Wenjiao1,2; Yin, Ronghua1,2; Zhou, Lutan1; Li, Zhongkun3; Wu, Mingyi1; Zhao, Jinhua1 | |
2018-07-15 | |
发表期刊 | CARBOHYDRATE RESEARCH |
ISSN | 0008-6215 |
卷号 | 464页码:12-18 |
摘要 | A fucan sulfate was isolated and purified from the sea cucumber Holothuria albiventer by papain enzymolysis, alkaline hydrolysis and ion-exchange chromatography. The water-soluble polysaccharide had high molecular weight and contained fucose and sulfate in a molar ratio of about 1:0.83. Methylation analysis of the native polysaccharide indicated that its glycosidic linkages and sulfate substituents might be at O-3 or O-3, 4 or O-2, 3, or O-2, 3, 4 positions. FT-IR and 2D NMR spectroscopies further revealed that the fucan sulfate is characteristically composed of a regular a (1 -> 3) linked hexasaccharide repeating unit which is substituted with sulfate esters in a distinctive pattern. Anticoagulant properties of the fucan sulfate and its depolymerized product were assessed in vitro in comparison with a low-molecular-weight heparin. The fucan sulfate exhibits strong APTT and TT prolonging activities and intrinsic factor Xase inhibitory activity, and its molecular size seemed to be required for these activities. |
关键词 | Fucan Sulfate Chemical Structure Anticoagulant Polysaccharide Sea Cucumber |
DOI | 10.1016/j.carres.2018.05.007 |
语种 | 英语 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/61368 |
专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Kunming Med Univ, Dept Pharm, Yanan Hosp, Kunming 650051, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Cai, Ying,Yang, Wenjiao,Yin, Ronghua,et al. An anticoagulant fucan sulfate with hexasaccharide repeating units from the sea cucumber Holothuria albiventer[J]. CARBOHYDRATE RESEARCH,2018,464:12-18. |
APA | Cai, Ying.,Yang, Wenjiao.,Yin, Ronghua.,Zhou, Lutan.,Li, Zhongkun.,...&Zhao, Jinhua.(2018).An anticoagulant fucan sulfate with hexasaccharide repeating units from the sea cucumber Holothuria albiventer.CARBOHYDRATE RESEARCH,464,12-18. |
MLA | Cai, Ying,et al."An anticoagulant fucan sulfate with hexasaccharide repeating units from the sea cucumber Holothuria albiventer".CARBOHYDRATE RESEARCH 464(2018):12-18. |
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