Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach
Zhao, Dake1,2,3; Shi, Yana4; Zhu, Xinyan4; Liu, Li4; Ji, Pengzhang4; Long, Chunlin5,6; Shen, Yong7; Kennelly, Edward J.5,8,9,10,11
2018-04-01
发表期刊PLANTA MEDICA
ISSN0032-0943
卷号84期号:6-7页码:434-441
摘要Despite their well-known toxicity, Aconitum species are important traditional medicines worldwide. Aconitum carmichaelii, known in Chinese as ?? (fuzi), is an officially recognized traditional Chinese medicine with characteristic analgesic and anti-inflammatory activities, whose principal pharmacological ingredients are considered as aconitine-type diterpene alkaloids. Notwithstanding the long-recorded use of A.carmichaelii in traditional Chinese medicine, no single-entity aconitum alkaloid drug has been developed for clinical use. UPLC-Q-TOF-MS was used to investigate the marker compounds that can be used to differentiate A.carmichaelii from seven other Aconitum species collected in Yunnan Province. Nontargeted principle component analysis scores plots found that all the tested Aconitum species clustered into three distinct groups, and A.carmichaelii was significantly different chemically than the other seven species. Furthermore, the primary and lateral roots of A.carmichaelii also showed significant differences. Using orthogonal partial least squares discriminate analysis analysis, eight marker compounds were identified, including 14-acetylkarakoline, aconitine, carmichaeline, fuziline, hypaconitine, mesaconitine, neoline, and talatisamine. Four of these aconitum alkaloids, fuziline, hypaconitine, mesaconitine, and neoline, showed significant analgesic activity in a dose-dependent manner compared to the negative and positive controls. However, hypaconitine, mesaconitine, and neoline exhibited significant acute toxicity activity, while fuziline showed no acute toxicity in mice, suggesting the relative safety of this alkaloid. This study provides a good example of how to differentiate an authentic medicinal plant from common adulterants using a metabolomics approach, and to identify compounds that may be developed into new drugs.
关键词Aconitum Aconitum Carmichaelii Ranunculaceae Aconitum Alkaloids Diterpene Alkaloids Analgesic Activity Acute Toxicity Traditional Chinese Medicine Tcm
DOI10.1055/s-0043-121708
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/61261
专题资源植物与生物技术所级重点实验室
作者单位1.Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources, Lab Ecol & Evolutionary Biol, Kunming, Yunnan, Peoples R China
2.Yunnan Baiyao Grp Co Ltd, Yunan Inst Mat Med, Kunming, Yunnan, Peoples R China
3.Chinese Acad Sci, Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming, Yunnan, Peoples R China
4.Yunnan Acad Agr Sci, Inst Med Plants, Kunming, Yunnan, Peoples R China
5.Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China
6.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
7.Yunnan Agr Univ, Coll Agr & Biotechnol, 452 Fengyuan Rd, Kunming 650201, Yunnan, Peoples R China
8.CUNY, Lehman Coll, Dept Biol Sci, 250 Bedford Pk Blvd W, Bronx, NY 10468 USA
9.CUNY, Grad Ctr, PhD Program Biochem, New York, NY USA
10.CUNY, Grad Ctr, PhD Program Biol, New York, NY USA
11.CUNY, Grad Ctr, PhD Program Chem, New York, NY USA
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Zhao, Dake,Shi, Yana,Zhu, Xinyan,et al. Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach[J]. PLANTA MEDICA,2018,84(6-7):434-441.
APA Zhao, Dake.,Shi, Yana.,Zhu, Xinyan.,Liu, Li.,Ji, Pengzhang.,...&Kennelly, Edward J..(2018).Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach.PLANTA MEDICA,84(6-7),434-441.
MLA Zhao, Dake,et al."Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach".PLANTA MEDICA 84.6-7(2018):434-441.
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