Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus
Qin, Yujing1; Yuan, Qingxia1; Zhang, Yuexing1; Li, Jialu1; Zhu, Xinjiao1; Zhao, Lingling1; Wen, Jing2; Liu, Jikai1; Zhao, Longyan1; Zhao, Jinhua1,3
2018-03-01
发表期刊MOLECULES
ISSN1420-3049
卷号23期号:3页码:590
摘要Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 +/- 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 degrees C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98C1 and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (Ga1NAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, Ga1NAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.
关键词Sea Cucumber Polysaccharide Enzyme-assisted Extraction Characterization Antioxidant Activity
DOI10.3390/molecules23030590
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/61254
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China
2.Lingnan Normal Univ, Coll Life Sci & Technol, Zhanjiang 524048, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
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Qin, Yujing,Yuan, Qingxia,Zhang, Yuexing,et al. Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus[J]. MOLECULES,2018,23(3):590.
APA Qin, Yujing.,Yuan, Qingxia.,Zhang, Yuexing.,Li, Jialu.,Zhu, Xinjiao.,...&Zhao, Jinhua.(2018).Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.MOLECULES,23(3),590.
MLA Qin, Yujing,et al."Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus".MOLECULES 23.3(2018):590.
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