Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus

doi:10.3390/molecules23030590

	Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus
	Qin, Yujing 1; Yuan, Qingxia 1; Zhang, Yuexing 1; Li, Jialu 1; Zhu, Xinjiao 1; Zhao, Lingling 1; Wen, Jing 2; Liu, Jikai 1; Zhao, Longyan1 ; Zhao, Jinhua1,3
	2018-03-01
发表期刊	MOLECULES
ISSN	1420-3049
卷号	23 期号:3 页码:590
摘要	Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 +/- 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 degrees C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98C1 and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (Ga1NAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, Ga1NAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.
关键词	Sea Cucumber Polysaccharide Enzyme-assisted Extraction Characterization Antioxidant Activity
DOI	10.3390/molecules23030590
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/61254
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China 2.Lingnan Normal Univ, Coll Life Sci & Technol, Zhanjiang 524048, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Qin, Yujing,Yuan, Qingxia,Zhang, Yuexing,et al. Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus[J]. MOLECULES,2018,23(3):590.
APA	Qin, Yujing.,Yuan, Qingxia.,Zhang, Yuexing.,Li, Jialu.,Zhu, Xinjiao.,...&Zhao, Jinhua.(2018).Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.MOLECULES,23(3),590.
MLA	Qin, Yujing,et al."Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus".MOLECULES 23.3(2018):590.

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