Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus
Qin, Yujing1; Yuan, Qingxia1; Zhang, Yuexing1; Li, Jialu1; Zhu, Xinjiao1; Zhao, Lingling1; Wen, Jing2; Liu, Jikai1; Zhao, Longyan1; Zhao, Jinhua1,3
2018-03-01
Source PublicationMOLECULES
ISSN1420-3049
Volume23Issue:3Pages:590
AbstractEnzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 +/- 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 degrees C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98C1 and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (Ga1NAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, Ga1NAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.
KeywordSea Cucumber Polysaccharide Enzyme-assisted Extraction Characterization Antioxidant Activity
DOI10.3390/molecules23030590
Language英语
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/61254
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China
2.Lingnan Normal Univ, Coll Life Sci & Technol, Zhanjiang 524048, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Qin, Yujing,Yuan, Qingxia,Zhang, Yuexing,et al. Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus[J]. MOLECULES,2018,23(3):590.
APA Qin, Yujing.,Yuan, Qingxia.,Zhang, Yuexing.,Li, Jialu.,Zhu, Xinjiao.,...&Zhao, Jinhua.(2018).Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.MOLECULES,23(3),590.
MLA Qin, Yujing,et al."Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus".MOLECULES 23.3(2018):590.
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