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题名: Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus
作者: Qin, Yujing1; Yuan, Qingxia1; Zhang, Yuexing1; Li, Jialu1; Zhu, Xinjiao1; Zhao, Lingling1; Wen, Jing2; Liu, Jikai1; Zhao, Longyan1; Zhao, Jinhua1, 3
刊名: MOLECULES
关键词: sea cucumber ; polysaccharide ; enzyme-assisted extraction ; characterization ; antioxidant activity
英文摘要: Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 +/- 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 degrees C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98C1 and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (Ga1NAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, Ga1NAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation.
出版日期: 2018-03-01
卷号: 23, 期号:3, 页码:590
DOI标识: 10.3390/molecules23030590
语种: 英语
ISSN号: 1420-3049
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/61254
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China
2.Lingnan Normal Univ, Coll Life Sci & Technol, Zhanjiang 524048, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China

Recommended Citation:
Qin, Yujing,Yuan, Qingxia,Zhang, Yuexing,et al. Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus[J]. MOLECULES,2018,23(3):590.
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