Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea
Wang, Qiuli1,2,3,4; Pu, Yanan1,2,3,5; Yang, Danni1,2,3,5; Yin, Xin1,2,3,5; He, Zhaorong4; Yang, Yunqiang1,2,3; Yang, Yongping1,2,3
2018-01-08
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
卷号495期号:2页码:1851-1857
摘要Reactive oxygen species (ROS) are a key factor in abiotic stresses; excess ROS is harmful to plants. Glutathione reductase (GR) plays an important role in scavenging ROS in plants. Here, a GR gene, named SpGR, was cloned from Stipa purpurea and characterized. The full-length open reading frame was 1497 bp, encoding 498 amino acids. Subcellular localization analysis indicated that SpGR was localized to both the plasma membrane and nucleus. The expression of SpGR was induced by cold, salt, and drought stresses. Functional analysis indicated that ectopic expression of SpGR in Arabidopsis thaliana resulted in greater tolerance to salt stress than that of wild-type plants, but no difference under cold or drought treatments. The results of GR activity and GSSG and GSH content analyses suggested that, under salt stress, transgenic plants produced more GR to reduce GSSG to GSH for scavenging ROS than wild-type plants. Therefore, SpGR may be a candidate gene for plants to resist abiotic stress. (C) 2017 Elsevier Inc. All rights reserved.
关键词Stipa Purpurea Glutathione Reductase Gssg Gsh Salinity
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/60566
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China
4.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Qiuli,Pu, Yanan,Yang, Danni,et al. Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(2):1851-1857.
APA Wang, Qiuli.,Pu, Yanan.,Yang, Danni.,Yin, Xin.,He, Zhaorong.,...&Yang, Yongping.(2018).Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(2),1851-1857.
MLA Wang, Qiuli,et al."Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.2(2018):1851-1857.
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