| Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS | |
Zhang, Jian-Gang1,2; Huang, Xiao-Yan1,2; Ma, Yun-Bao1,2; Zhang, Xue-Mei1,2; Chen, Ji-Jun1,2 ; Geng, Chang-An1,2
| |
| 2018-04-01 | |
| 发表期刊 | JOURNAL OF SEPARATION SCIENCE
 |
| ISSN | 1615-9306 |
| 卷号 | 41期号:7页码:1532-1538 |
| 摘要 | Uncaria rhynchophylla (Gou-Teng) as the monarch herb of many formulae (Fufang), e.g. "Tian-Ma-Gou-Teng-Yin," "Ling-Jiao-Gou-Teng-Yin," and "Yi-Gan-San", is a famous traditional Chinese medicine documented in the Chinese pharmacopoeia for mental and cardiovascular diseases. In the traditional Chinese medicine system, only the hook-bearing stems are used as the crude materials for Gou-Teng, and the hooks are always considered more effective than the stems. Focusing on the mono-herb and its active constituents from combinatorial formulae is the core idea of reductionism of traditional Chinese medicine theory. Detailed liquid chromatography with mass spectrometry analysis on the hooks of U. rhynchophylla was performed to profile the chemical constituents based on tandem mass spectrometry fragmentation and UV absorption. Under the guidance of liquid chromatography with ion trap/time-of-flight mass spectrometry, one new indole alkaloid triglycoside (1), together with five known compounds 2-6 as the main constituents, were isolated from the hooks of U. rhynchophylla by various column chromatography methods. Compound 1 showed moderate activity on MT1 and MT2 melatonin receptors with agonistic rates of 79.6 and 46.3% at the concentration of 1 mM. This dereplication strategy can be equally applicable to rapidly disclose the active constituents of other Chinese herbs through targeted purification. |
| 关键词 | 5-hydroxytryptamine Receptors Dereplication-guided Isolation Indole Alkaloid Triglycoside Melatonin Receptors Traditional Chinese Medicine |
| DOI | 10.1002/jssc.201701175 |
| 语种 | 英语 |
| WOS记录号 | WOS:000429715600003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/60499 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China 2.Yunnan Key Lab Nat Med Chem, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,et al. Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS[J]. JOURNAL OF SEPARATION SCIENCE,2018,41(7):1532-1538. |
| APA | Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,Zhang, Xue-Mei,Chen, Ji-Jun,&Geng, Chang-An.(2018).Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS.JOURNAL OF SEPARATION SCIENCE,41(7),1532-1538. |
| MLA | Zhang, Jian-Gang,et al."Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS".JOURNAL OF SEPARATION SCIENCE 41.7(2018):1532-1538. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| zhang2017.pdf（1155KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论