Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS
Zhang, Jian-Gang1,2; Huang, Xiao-Yan1,2; Ma, Yun-Bao1,2; Zhang, Xue-Mei1,2; Chen, Ji-Jun1,2; Geng, Chang-An1,2
2018-04-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume41Issue:7Pages:1532-1538
AbstractUncaria rhynchophylla (Gou-Teng) as the monarch herb of many formulae (Fufang), e.g. "Tian-Ma-Gou-Teng-Yin," "Ling-Jiao-Gou-Teng-Yin," and "Yi-Gan-San", is a famous traditional Chinese medicine documented in the Chinese pharmacopoeia for mental and cardiovascular diseases. In the traditional Chinese medicine system, only the hook-bearing stems are used as the crude materials for Gou-Teng, and the hooks are always considered more effective than the stems. Focusing on the mono-herb and its active constituents from combinatorial formulae is the core idea of reductionism of traditional Chinese medicine theory. Detailed liquid chromatography with mass spectrometry analysis on the hooks of U. rhynchophylla was performed to profile the chemical constituents based on tandem mass spectrometry fragmentation and UV absorption. Under the guidance of liquid chromatography with ion trap/time-of-flight mass spectrometry, one new indole alkaloid triglycoside (1), together with five known compounds 2-6 as the main constituents, were isolated from the hooks of U. rhynchophylla by various column chromatography methods. Compound 1 showed moderate activity on MT1 and MT2 melatonin receptors with agonistic rates of 79.6 and 46.3% at the concentration of 1 mM. This dereplication strategy can be equally applicable to rapidly disclose the active constituents of other Chinese herbs through targeted purification.
Keyword5-hydroxytryptamine Receptors Dereplication-guided Isolation Indole Alkaloid Triglycoside Melatonin Receptors Traditional Chinese Medicine
DOI10.1002/jssc.201701175
Language英语
WOS IDWOS:000429715600003
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/60499
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China
2.Yunnan Key Lab Nat Med Chem, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,et al. Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS[J]. JOURNAL OF SEPARATION SCIENCE,2018,41(7):1532-1538.
APA Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,Zhang, Xue-Mei,Chen, Ji-Jun,&Geng, Chang-An.(2018).Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS.JOURNAL OF SEPARATION SCIENCE,41(7),1532-1538.
MLA Zhang, Jian-Gang,et al."Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS".JOURNAL OF SEPARATION SCIENCE 41.7(2018):1532-1538.
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