Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS

doi:10.1002/jssc.201701175

	Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS
	Zhang, Jian-Gang 1,2; Huang, Xiao-Yan 1,2; Ma, Yun-Bao 1,2; Zhang, Xue-Mei 1,2; Chen, Ji-Jun1,2 ; Geng, Chang-An1,2
	2018-04-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
卷号	41 期号:7 页码:1532-1538
摘要	Uncaria rhynchophylla (Gou-Teng) as the monarch herb of many formulae (Fufang), e.g. "Tian-Ma-Gou-Teng-Yin," "Ling-Jiao-Gou-Teng-Yin," and "Yi-Gan-San", is a famous traditional Chinese medicine documented in the Chinese pharmacopoeia for mental and cardiovascular diseases. In the traditional Chinese medicine system, only the hook-bearing stems are used as the crude materials for Gou-Teng, and the hooks are always considered more effective than the stems. Focusing on the mono-herb and its active constituents from combinatorial formulae is the core idea of reductionism of traditional Chinese medicine theory. Detailed liquid chromatography with mass spectrometry analysis on the hooks of U. rhynchophylla was performed to profile the chemical constituents based on tandem mass spectrometry fragmentation and UV absorption. Under the guidance of liquid chromatography with ion trap/time-of-flight mass spectrometry, one new indole alkaloid triglycoside (1), together with five known compounds 2-6 as the main constituents, were isolated from the hooks of U. rhynchophylla by various column chromatography methods. Compound 1 showed moderate activity on MT1 and MT2 melatonin receptors with agonistic rates of 79.6 and 46.3% at the concentration of 1 mM. This dereplication strategy can be equally applicable to rapidly disclose the active constituents of other Chinese herbs through targeted purification.
关键词	5-hydroxytryptamine Receptors Dereplication-guided Isolation Indole Alkaloid Triglycoside Melatonin Receptors Traditional Chinese Medicine
DOI	10.1002/jssc.201701175
语种	英语
WOS记录号	WOS:000429715600003
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/60499
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China 2.Yunnan Key Lab Nat Med Chem, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,et al. Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS[J]. JOURNAL OF SEPARATION SCIENCE,2018,41(7):1532-1538.
APA	Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,Zhang, Xue-Mei,Chen, Ji-Jun,&Geng, Chang-An.(2018).Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS.JOURNAL OF SEPARATION SCIENCE,41(7),1532-1538.
MLA	Zhang, Jian-Gang,et al."Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS".JOURNAL OF SEPARATION SCIENCE 41.7(2018):1532-1538.

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