Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS
Zhang, Jian-Gang1,2; Huang, Xiao-Yan1,2; Ma, Yun-Bao1,2; Zhang, Xue-Mei1,2; Chen, Ji-Jun1,2; Geng, Chang-An1,2
2018-04-01
发表期刊JOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
卷号41期号:7页码:1532-1538
摘要Uncaria rhynchophylla (Gou-Teng) as the monarch herb of many formulae (Fufang), e.g. "Tian-Ma-Gou-Teng-Yin," "Ling-Jiao-Gou-Teng-Yin," and "Yi-Gan-San", is a famous traditional Chinese medicine documented in the Chinese pharmacopoeia for mental and cardiovascular diseases. In the traditional Chinese medicine system, only the hook-bearing stems are used as the crude materials for Gou-Teng, and the hooks are always considered more effective than the stems. Focusing on the mono-herb and its active constituents from combinatorial formulae is the core idea of reductionism of traditional Chinese medicine theory. Detailed liquid chromatography with mass spectrometry analysis on the hooks of U. rhynchophylla was performed to profile the chemical constituents based on tandem mass spectrometry fragmentation and UV absorption. Under the guidance of liquid chromatography with ion trap/time-of-flight mass spectrometry, one new indole alkaloid triglycoside (1), together with five known compounds 2-6 as the main constituents, were isolated from the hooks of U. rhynchophylla by various column chromatography methods. Compound 1 showed moderate activity on MT1 and MT2 melatonin receptors with agonistic rates of 79.6 and 46.3% at the concentration of 1 mM. This dereplication strategy can be equally applicable to rapidly disclose the active constituents of other Chinese herbs through targeted purification.
关键词5-hydroxytryptamine Receptors Dereplication-guided Isolation Indole Alkaloid Triglycoside Melatonin Receptors Traditional Chinese Medicine
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/60499
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China
2.Yunnan Key Lab Nat Med Chem, Kunming, Yunnan, Peoples R China
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Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,et al. Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS[J]. JOURNAL OF SEPARATION SCIENCE,2018,41(7):1532-1538.
APA Zhang, Jian-Gang,Huang, Xiao-Yan,Ma, Yun-Bao,Zhang, Xue-Mei,Chen, Ji-Jun,&Geng, Chang-An.(2018).Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS.JOURNAL OF SEPARATION SCIENCE,41(7),1532-1538.
MLA Zhang, Jian-Gang,et al."Dereplication-guided isolation of a new indole alkaloid triglycoside from the hooks of Uncaria rhynchophylla by LC with ion trap time-of-flight MS".JOURNAL OF SEPARATION SCIENCE 41.7(2018):1532-1538.
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