Metabolic profiling of myrislignan by UPLC-ESI-QTOFMS-based metabolomics
Yang, Xiao-Nan1; Lv, Qian-Qian1,2; Zhao, Qi1,3; Li, Xin-Mei1; Yan, Dong-Mei4; Yang, Xiu-Wei5; Li, Fei1
2017
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume7Issue:64Pages:40131-40140
AbstractMyrislignan (MRL) is a bioactive 8-O-4'-neolignan distributed in nutmeg that is an official Traditional Chinese Medicine used for the treatment of gastrointestinal diseases in China. In the present study, the metabolic map of MRL was determined in mouse by ultra-performance chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS)-based metabolomics. A total of 23 MRL metabolites (19 were newly identified) were determined in both in vivo and in vitro metabolism of MRL. It was found that hydroxylation and demethylation were the major metabolic pathways for MRL metabolism in vitro and in vivo, respectively. Recombinant cytochrome P450s (CYPs) screening revealed that CYP3A4 and CYP3A5 played a crucial role in the metabolism of MRL. Interestingly, MRL and its metabolites were prone to generate the high abundance ions of Na+ adducts due to one 8-O-4' type bond in their molecular structures. Taken together, these data reveal the metabolic map of MRL, contributing to the understanding of the metabolism and disposition of 8-O-4'-neolignans in nutmeg.
KeywordMyristica-fragrans In-vitro Liver-microsomes Neolignans Extract Lignans Pharmacokinetics Identification Noscapine Reveals
Subject AreaChemistry, Multidisciplinary
DOI10.1039/c7ra06189g
Indexed BySCI
Language英语
WOS IDWOS:000408043100011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/54976
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
2.Jiangxi Univ Tradit Chinese Med, Res Ctr Differentiat & Dev Basic Theory Tradit Ch, Nanchang 330004, Jiangxi, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Jiangxi Univ Tradit Chinese Med, Sch Pharm, Nanchang 330004, Jiangxi, Peoples R China
5.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Yang, Xiao-Nan,Lv, Qian-Qian,Zhao, Qi,et al. Metabolic profiling of myrislignan by UPLC-ESI-QTOFMS-based metabolomics[J]. RSC ADVANCES,2017,7(64):40131-40140.
APA Yang, Xiao-Nan.,Lv, Qian-Qian.,Zhao, Qi.,Li, Xin-Mei.,Yan, Dong-Mei.,...&Li, Fei.(2017).Metabolic profiling of myrislignan by UPLC-ESI-QTOFMS-based metabolomics.RSC ADVANCES,7(64),40131-40140.
MLA Yang, Xiao-Nan,et al."Metabolic profiling of myrislignan by UPLC-ESI-QTOFMS-based metabolomics".RSC ADVANCES 7.64(2017):40131-40140.
Files in This Item: Download All
File Name/Size DocType Version Access License
10.1039@c7ra06189g.p(956KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Xiao-Nan]'s Articles
[Lv, Qian-Qian]'s Articles
[Zhao, Qi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Xiao-Nan]'s Articles
[Lv, Qian-Qian]'s Articles
[Zhao, Qi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Xiao-Nan]'s Articles
[Lv, Qian-Qian]'s Articles
[Zhao, Qi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 10.1039@c7ra06189g.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.