Comparative transcriptome analyses of a late-maturing mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit maturation
Wang,Lu; Hua,Qingzhu; Ma,Yuewen; Hu,Guibing; Qin,Yonghua
2017
发表期刊PEERJ
ISSN2167-8359
卷号5页码:e3343
摘要Characteristics of late maturity in fruit are good agronomic traits for extending the harvest period and marketing time. However, underlying molecular basis of the late-maturing mechanism in fruit is largely unknown. In this study, RNA sequencing (RNA-Seq) technology was used to identify differentially expressed genes (DEGs) related to late-maturing characteristics from a late-maturing mutant 'Huawan Wuzishatangiu) (HWWZSTJ) (Citrus reticulata Blanco) and its original line 'Wuzishatangju) (WZSTJ). A total of approximately 17.0 Gb and 84.2 M paried-end reads were obtained DEGs were significantly enriched in the pathway of photosynthesis, phenylpropanoid biosynthesis, carotenoid biosynthesis, chlorophyll and abscisic acid (ABA) metabolism. Thirteen candidate transcripts related to chlorophyll metabolism, carotenoid biosynthesis and ABA metabolism were analyzed using real-time quantitative PCR (qPCR) at all fruit maturing stages of HWWZSTJ and WZSTJ. Chlorophyllase (CLH) and divinyl reductase (DVR). from chlorophyll metabolism, phytoene synthase (PSY) and capsanthin/capsorubin synthase (CCS) from carotenoid biosynthesis, and abscisic acid 8 '-hydroxylasea (AB1) and 9-cis-epoxycarotenoid dioxygenase(NCED1) from ABA metabolism were cloned and analyzed. The expression pattern of NCED1 indicated its role in the late-maturing characteristics of HWWZSTJ. There were 270 consecutive bases missing in HWWZSTJ in comparison with full-length sequences of NCED1 cDNA from WZSTJ. Those results suggested that NCED1 might play an important role in the late maturity of HWWZSTJ. This study provides new information on complex process that results in the late maturity of Citrus fruit at the transcriptional level.
关键词Abscisic-acid Biosynthesis Key Enzyme 9-cis-epoxycarotenoid Dioxygenase Phytoene Synthase Tomato Fruit Aba Ethylene Cloning Chlorophyllase Modulation Citrus Reticulata Blanco Late Maturity Rna-seq Gene Expression Ncedi
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/54952
专题植物化学与西部植物资源持续利用国家重点实验室
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Wang,Lu,Hua,Qingzhu,Ma,Yuewen,et al. Comparative transcriptome analyses of a late-maturing mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit maturation[J]. PEERJ,2017,5:e3343.
APA Wang,Lu,Hua,Qingzhu,Ma,Yuewen,Hu,Guibing,&Qin,Yonghua.(2017).Comparative transcriptome analyses of a late-maturing mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit maturation.PEERJ,5,e3343.
MLA Wang,Lu,et al."Comparative transcriptome analyses of a late-maturing mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit maturation".PEERJ 5(2017):e3343.
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