| Anti-TMV activity and functional mechanisms of two sesquiterpenoids isolated from Tithonia diversifolia | |
| Zhao, Lihua1,2; Dong, Jiahong2; Hu, Zhonghui3; Li, Shunlin3; Su, Xiaoxia2; Zhang, Jie2; Yin, Yueyan2; Xu, Tao2; Zhang, Zhongkai2; Chen, Hairu1 | |
| 2017-08-01 | |
| 发表期刊 | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
 |
| ISSN | 0048-3575 |
| 卷号 | 140页码:24-29 |
| 摘要 | Unlike chemical pesticides, antiviral plants are biodegradable, replenishable and safe. In this study, 14 sesquiterpene compounds from Tithonia diversifolia were tested for their activities against Tobacco mosaic virus (TMV) using the half-leaf method. Tagitinin C (Ses-2) and 1 beta-methoxydiversifolin-3-0-methyl ether (Ses-5) were found to have in vivo curative activities of 62.86% and 60.27% respectively, at concentrations of 100 mu g/mL, respectively. In contrast, the in vivo curative inhibition rate of control agent ningnanmycin was 52.48%. Indirect enzyme-linked immunosorbent assay (ID-ELISA) also verified Ses-2 and Ses-5 had higher inhibition activities than the control agent ningnanmycin. Additionally, qRT-PCR showed that both Ses-2 and Ses-5 can partly inhibit the expression of CP and RdRp, two genes that play key roles in TMV infection. When TMV started to systemically spread, Ses-2 inhibited CP expression while Ses-5 inhibited RdRp expression. These results suggest that the two bio-agents have anti-TMV activities and may be used as bio-pesticides to control the plant virus. 2017 The Authors. Published by Elsevier Inc. |
| 关键词 | Curative Activities Sesquiterpene Half-leaf Method Bio-pesticides |
| 学科领域 | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| DOI | 10.1016/j.pestbp.2017.05.009 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000407533100004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/54937 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Kunming 650204, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lihua,Dong, Jiahong,Hu, Zhonghui,et al. Anti-TMV activity and functional mechanisms of two sesquiterpenoids isolated from Tithonia diversifolia[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2017,140:24-29. |
| APA | Zhao, Lihua.,Dong, Jiahong.,Hu, Zhonghui.,Li, Shunlin.,Su, Xiaoxia.,...&Chen, Hairu.(2017).Anti-TMV activity and functional mechanisms of two sesquiterpenoids isolated from Tithonia diversifolia.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,140,24-29. |
| MLA | Zhao, Lihua,et al."Anti-TMV activity and functional mechanisms of two sesquiterpenoids isolated from Tithonia diversifolia".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 140(2017):24-29. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| zhao2017.pdf（517KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论