Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation

doi:10.3389/fpls.2017.01738

	Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation
	Song, Juan 1,2; Liu, Hui 1; Zhuang, Huifu1,2 ; Zhao, Chunxia 3; Xu, Yuxing 1,2; Wu, Shibo 4; Qi, Jinfeng 1; Li, Jing1 ; Hettenhausen, Christian 1; Wu, Jianqiang1
	2017-10-10
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	8 页码:1738
摘要	Maize (Zea mays L.) is a staple crop worldwide with extensive genetic variations. Various insects attack maize plants causing large yield loss. Here, we investigated the responses of maize B73, a susceptible line, and Mo17, a resistant line, to the aphid Rhopalosiphum padi on metabolite and transcriptome levels. R. padi feeding had no effect on the levels of the defensive metabolites benzoxazinoids (Bxs) in either line, and Mo17 contained substantially greater levels of Bxs than did B73. Profiling of the differentially expressed genes revealed that B73 and Mo17 responded to R. padi infestation specifically, and importantly, these two lines showed large gene expression differences even without R. padi herbivory. Correlation analysis identified four transcription factors (TFs) that might account for the high Bx levels in Mo17. Similarly, genome-wide alternative splicing (AS) analyses indicated that both B73 and Mo17 had temporally specific responses to R. padi infestation, and these two lines also exhibited large differences of AS regulation under normal condition, and 340 genes, including 10 TFs, were constantly differentially spliced. This study provides large-scale resource datasets for further studies on the mechanisms underlying maize-aphid interactions, and highlights the phenotypic divergence in defense against aphids among maize varieties.
关键词	Maize (Zea Mays L.) Rhopalosiphum Padi Transcriptome Alternative Splicing Metabolites Benzoxazinoid
学科领域	Plant Sciences
DOI	10.3389/fpls.2017.01738
收录类别	SCI
语种	英语
WOS记录号	WOS:000412627200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/54871
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 4.Yunnan Acad Sci & Technol Dev, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Song, Juan,Liu, Hui,Zhuang, Huifu,et al. Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation[J]. FRONTIERS IN PLANT SCIENCE,2017,8:1738.
APA	Song, Juan.,Liu, Hui.,Zhuang, Huifu.,Zhao, Chunxia.,Xu, Yuxing.,...&Wu, Jianqiang.(2017).Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation.FRONTIERS IN PLANT SCIENCE,8,1738.
MLA	Song, Juan,et al."Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation".FRONTIERS IN PLANT SCIENCE 8(2017):1738.

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