Metabolic profiling of dehydrodiisoeugenol using xenobiotic metabolomics
Lv, Qian-Qian1,2; Yang, Xiao-Nan1; Yan, Dong-Mei3; Liang, Wei-Qing4; Liu, Hong-Ning2; Yang, Xiu-Wei5; Li, Fei1,2
2017-10-25
发表期刊JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
卷号145页码:725-733
摘要Dehydrodiisoeugenol (DDIE), a representative and major benzofuran-type neolignan in Myristicafragrans Houtt., shows anti-inflammatory and anti-bacterial actions. In order to better understand its pharmacological properties, xenobiotic metabolomics was used to determine the metabolic map of DDIE and its influence on endogenous metabolites. Total thirteen metabolites of DDIE were identified through in vivo and in vitro metabolism, and seven of them were reported for the first time in the present study. The identity of DDIE metabolites was achieved by comparison of the MS/MS fragmentation pattern with DDIE using ultra-performance chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI- QTOPMS). Demethylation and ring-opening reaction were the major metabolic pathways for in vivo metabolism of DDIE. Recombinant cytochrome P450 s (CYPs) screening revealed that CYP1A1 is a primary enzyme contributing to the formation of metabolites D1-D4. More importantly, the levels of two endogenous metabolites 2,8-dihydroxyquinoline and its glucuronide were significantly elevated in mouse urine after DDIE exposure, which explains in part its modulatory effects on gut micro biota. Taken together, these data contribute to the understanding of the disposition and pharmacological activities of DDIE in vivo. (C) 2017 Elsevier B.V. All rights reserved.
关键词Dehydrodiisoeugenol Xenobiotic Metabolomics Metabolic Profiling Uplc-esi-qtofms
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/54867
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Jiangxi Univ Tradit Chinese Med, Res Ctr Differentiat & Dev Basic Theory Tradit Ch, Nanchang 330004, Jiangxi, Peoples R China
3.Jiangxi Univ Tradit Chinese Med, Sch Pharm, Nanchang 330004, Jiangxi, Peoples R China
4.Zhejiang Acad Tradit Chinese Med, Ctr Med Resources Res, Hangzhou 310007, Zhejiang, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
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Lv, Qian-Qian,Yang, Xiao-Nan,Yan, Dong-Mei,et al. Metabolic profiling of dehydrodiisoeugenol using xenobiotic metabolomics[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,145:725-733.
APA Lv, Qian-Qian.,Yang, Xiao-Nan.,Yan, Dong-Mei.,Liang, Wei-Qing.,Liu, Hong-Ning.,...&Li, Fei.(2017).Metabolic profiling of dehydrodiisoeugenol using xenobiotic metabolomics.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,145,725-733.
MLA Lv, Qian-Qian,et al."Metabolic profiling of dehydrodiisoeugenol using xenobiotic metabolomics".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 145(2017):725-733.
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