Isolation and characterization of cytotoxic cyclotides from Viola philippica
He, Wenjun1,3; Chan, Lai Yue2; Zeng, Guangzhi1; Daly, Norelle L.2; Craik, David J.2; Tan, Ninghua1
Corresponding AuthorTan, NH (reprint author), Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, 132 Lanhei Rd, Kunming 650204, Yunnan, Peoples R China
2011-08-01
Source PublicationPEPTIDES
ISSN0196-9781
Volume32Issue:8Pages:1719-1723
AbstractCyclotides are a large family of plant peptides characterized by a macrocyclic backbone and knotted arrangement of three disulfide bonds. This unique structure renders cyclotides exceptionally stable to thermal, chemical and enzymatic treatments. They exhibit a variety of bioactivities, including uterotonic, anti-HIV, cytotoxic and hemolytic activity and it is these properties that make cyclotides an interesting peptide scaffold for drug design. In this study, eight new cyclotides (Viphi A-H), along with eight known cyclotides, were isolated from Viola philippica, a plant from the Violaceae family. In addition, Viba 17 and Mram 8 were isolated for the first time as peptides. The sequences of these cyclotides were elucidated primarily by using a strategy involving reduction, enzymatic digestion and tandem mass spectroscopy sequencing. Several of the cyclotides showed cytotoxic activities against the cancer cell lines MM96L, HeLa and BGC-823. The novel cyclotides reported here: (1) enhance the known sequence variation observed for cyclotides; (2) extend the number of species known to contain cyclotides; (3) provide interesting structure-activity relationships that delineate residues important for cytotoxic activity. In addition, this study provides insights into the potential active ingredients of traditional Chinese medicines. (C) 2011 Elsevier Inc. All rights reserved.
KeywordCyclotides Cyclic Peptides Violaceae Viola Philippica Cytotoxic Activity Viphi A-h
Subject AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy
DOI10.1016/j.peptides.2011.06.016
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS SubjectBiochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS IDWOS:000294572800021
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/5357
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
He, Wenjun,Chan, Lai Yue,Zeng, Guangzhi,et al. Isolation and characterization of cytotoxic cyclotides from Viola philippica[J]. PEPTIDES,2011,32(8):1719-1723.
APA He, Wenjun,Chan, Lai Yue,Zeng, Guangzhi,Daly, Norelle L.,Craik, David J.,&Tan, Ninghua.(2011).Isolation and characterization of cytotoxic cyclotides from Viola philippica.PEPTIDES,32(8),1719-1723.
MLA He, Wenjun,et al."Isolation and characterization of cytotoxic cyclotides from Viola philippica".PEPTIDES 32.8(2011):1719-1723.
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