Isolation and characterization of cytotoxic cyclotides from Viola philippica
He, Wenjun1,3; Chan, Lai Yue2; Zeng, Guangzhi1; Daly, Norelle L.2; Craik, David J.2; Tan, Ninghua1
2011-08-01
发表期刊PEPTIDES
ISSN0196-9781
卷号32期号:8页码:1719-1723
摘要Cyclotides are a large family of plant peptides characterized by a macrocyclic backbone and knotted arrangement of three disulfide bonds. This unique structure renders cyclotides exceptionally stable to thermal, chemical and enzymatic treatments. They exhibit a variety of bioactivities, including uterotonic, anti-HIV, cytotoxic and hemolytic activity and it is these properties that make cyclotides an interesting peptide scaffold for drug design. In this study, eight new cyclotides (Viphi A-H), along with eight known cyclotides, were isolated from Viola philippica, a plant from the Violaceae family. In addition, Viba 17 and Mram 8 were isolated for the first time as peptides. The sequences of these cyclotides were elucidated primarily by using a strategy involving reduction, enzymatic digestion and tandem mass spectroscopy sequencing. Several of the cyclotides showed cytotoxic activities against the cancer cell lines MM96L, HeLa and BGC-823. The novel cyclotides reported here: (1) enhance the known sequence variation observed for cyclotides; (2) extend the number of species known to contain cyclotides; (3) provide interesting structure-activity relationships that delineate residues important for cytotoxic activity. In addition, this study provides insights into the potential active ingredients of traditional Chinese medicines. (C) 2011 Elsevier Inc. All rights reserved.
关键词Cyclotides Cyclic Peptides Violaceae Viola Philippica Cytotoxic Activity Viphi A-h
资助信息NSFC, ARC; National Natural Science Foundation of China[30725048, 91013002, U1032602]; National New Drug Innovation Great Project of China[2011ZX09307-002-02]; National Basic Research Program of China[2009C8522300]; China Scholarship Council
学科领域Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
DOI10.1016/j.peptides.2011.06.016
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS类目Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS记录号WOS:000294572800021
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/5357
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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He, Wenjun,Chan, Lai Yue,Zeng, Guangzhi,et al. Isolation and characterization of cytotoxic cyclotides from Viola philippica[J]. PEPTIDES,2011,32(8):1719-1723.
APA He, Wenjun,Chan, Lai Yue,Zeng, Guangzhi,Daly, Norelle L.,Craik, David J.,&Tan, Ninghua.(2011).Isolation and characterization of cytotoxic cyclotides from Viola philippica.PEPTIDES,32(8),1719-1723.
MLA He, Wenjun,et al."Isolation and characterization of cytotoxic cyclotides from Viola philippica".PEPTIDES 32.8(2011):1719-1723.
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