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题名: Isolation and characterization of cytotoxic cyclotides from Viola philippica
作者: He, Wenjun1, 3; Chan, Lai Yue2; Zeng, Guangzhi1; Daly, Norelle L.2; Craik, David J.2; Tan, Ninghua1
刊名: PEPTIDES
关键词: Cyclotides ; Cyclic peptides ; Violaceae ; Viola philippica ; Cytotoxic activity ; Viphi A-H
英文摘要: Cyclotides are a large family of plant peptides characterized by a macrocyclic backbone and knotted arrangement of three disulfide bonds. This unique structure renders cyclotides exceptionally stable to thermal, chemical and enzymatic treatments. They exhibit a variety of bioactivities, including uterotonic, anti-HIV, cytotoxic and hemolytic activity and it is these properties that make cyclotides an interesting peptide scaffold for drug design. In this study, eight new cyclotides (Viphi A-H), along with eight known cyclotides, were isolated from Viola philippica, a plant from the Violaceae family. In addition, Viba 17 and Mram 8 were isolated for the first time as peptides. The sequences of these cyclotides were elucidated primarily by using a strategy involving reduction, enzymatic digestion and tandem mass spectroscopy sequencing. Several of the cyclotides showed cytotoxic activities against the cancer cell lines MM96L, HeLa and BGC-823. The novel cyclotides reported here: (1) enhance the known sequence variation observed for cyclotides; (2) extend the number of species known to contain cyclotides; (3) provide interesting structure-activity relationships that delineate residues important for cytotoxic activity. In addition, this study provides insights into the potential active ingredients of traditional Chinese medicines. (C) 2011 Elsevier Inc. All rights reserved.
出版日期: 2011-08-01
卷号: 32, 期号:8, 页码:1719-1723
DOI标识: 10.1016/j.peptides.2011.06.016
语种: 英语
ISSN号: 0196-9781
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/5357
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
He, WJ; Chan, LY; Zeng, GZ; Daly, NL; Craik, DJ; Tan, NH.Isolation and characterization of cytotoxic cyclotides from Viola philippica,PEPTIDES,2011,32(8):1719-1723
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