The protective role of 5-HMF against hypoxic injury
Li, Ming-Ming1,2; Wu, Li-Ying1; Zhao, Tong1; Xiong, Lei1; Huang, Xin1; Liu, Zhao-Hui1; Fan, Xue-Lai1; Xiao, Cheng-Rong3; Gao, Yue3; Ma, Yun-Bao4; Chen, Ji-Jun4; Zhu, Ling-Ling1; Fan, Ming1,2
Corresponding AuthorZhu, LL (reprint author), Inst Basic Med Sci, Dept Brain Protect & Plast, 27 Taiping Rd, Beijing 100850, Peoples R China
AbstractIn an attempt to find new types of anti-hypoxic agents from herbs, we identified 5-hydroxymethyl-2-furfural (5-HMF) as a natural agent that fulfills the criterion. 5-HMF, the final product of carbohydrate metabolism, has favorable biological effects such as anti-oxidant activity and inhibiting sickling of red blood cells. The role of 5-HMF in hypoxia, however, is not yet. Our pilot results showed that pretreatment with 5-HMF markedly increased both the survival time and the survival rate of mice under hypoxic stress. The present study was aimed to further investigate the protective role of 5-HMF and the underlying mechanisms in hypoxic injury using ECV304 cells as an in vitro model. ECV304 cells pretreated with or without 5-HMF for 1 h were exposed to hypoxic condition (0.3% O-2) for 24 h and then cell apoptosis, necrosis, the changes of mitochondrial membrane potential (MMP) and the expressions of phosphorylation- extracellular signal-regulated kinase (p-ERK) were investigated. Pretreatment with 5-HMF markedly attenuated hypoxia-induced cell necrosis and apoptosis at late stage (p < 0.01). Furthermore, pretreatment with 5-HMF rescued both the decline of the MMP and the increase of p-ERK protein under hypoxia. In a word, these results indicated that 5-HMF had protective effects against hypoxic injury in ECV304 cells, and its effects on MMP and p-ERK may be involved in the mechanism.
Keyword5-hmf Hypoxic Injury Ecv304 Cells Mmp
Subject AreaCell Biology
Indexed BySCI
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000289208300004
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Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Inst Basic Med Sci, Dept Brain Protect & Plast, Beijing 100850, Peoples R China
2.Capital Med Univ, Sch Basic Med Sci, Beijing Inst Neurosci, Beijing 100069, Peoples R China
3.Beijing Inst Radiat Med, Beijing 100850, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources Western, Kunming 650204, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Li, Ming-Ming,Wu, Li-Ying,Zhao, Tong,et al. The protective role of 5-HMF against hypoxic injury[J]. CELL STRESS & CHAPERONES,2011,16(3):267-273.
APA Li, Ming-Ming.,Wu, Li-Ying.,Zhao, Tong.,Xiong, Lei.,Huang, Xin.,...&Fan, Ming.(2011).The protective role of 5-HMF against hypoxic injury.CELL STRESS & CHAPERONES,16(3),267-273.
MLA Li, Ming-Ming,et al."The protective role of 5-HMF against hypoxic injury".CELL STRESS & CHAPERONES 16.3(2011):267-273.
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