Characterization of a chestnut FLORICAULA/LEAFY homologous gene | |
Liu, Tao1; Hu, Yun-qian2; Li, Xiao-xian2 | |
通讯作者 | Liu, T (reprint author), Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China |
2011-05-16 | |
发表期刊 | AFRICAN JOURNAL OF BIOTECHNOLOGY |
ISSN | 1684-5315 |
卷号 | 10期号:20页码:3978-3985 |
摘要 | The FLORICAULA/LEAFY (FLO/LFY) homologues' genes are necessary for normal flower development and play a key role in diverse angiosperm species. In this paper, an orthologue of FLORICAULA/LEAFY, CmLFY (chestnut FLO/LFY), was isolated from the inflorescence of chestnut trees. Its expression was detected in various tissues. Furthermore, the flowering effectiveness of the gene was assessed with transgenic Arabidopsis. CmLFY protein showed a high degree of similarity to PEAFLO (78%), which is a homologue of FLO/LFY from pea. RT-PCR analysis showed that, CmLFY expressed at high levels in inflorescences, but not in young leaves, fruits or stems. The transgenic Arabidopsis with over-expressed CmLFY showed accelerated flowering, which supports that CmLFY encodes a functional orthologue of the FLORICAULA/LEAFY genes of angiosperms despite its sequence divergence. These results suggest that CmLFY is involved in inflorescence development in chestnut. |
关键词 | Chestnut Homologue Floricaula/leafy |
学科领域 | Biotechnology & Applied Microbiology |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000294612200004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/5309 |
专题 | 资源植物与生物技术所级重点实验室 |
作者单位 | 1.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Tao,Hu, Yun-qian,Li, Xiao-xian. Characterization of a chestnut FLORICAULA/LEAFY homologous gene[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(20):3978-3985. |
APA | Liu, Tao,Hu, Yun-qian,&Li, Xiao-xian.(2011).Characterization of a chestnut FLORICAULA/LEAFY homologous gene.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(20),3978-3985. |
MLA | Liu, Tao,et al."Characterization of a chestnut FLORICAULA/LEAFY homologous gene".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.20(2011):3978-3985. |
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