Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)

doi:10.1016/j.plantsci.2010.12.006

	Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)
	Liu, Li-Qin 1,2; Gu, Zhi-Jian 1
通讯作者	Liu, LQ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
	2011-03-01
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	180 期号:3 页码:554-559
摘要	Camellia reticulata (Theaceae genus Camellia) is a world-famous, ornamental flowering plant. More interestingly, it has a polyploid series varying from 2n = 2x = 30, 2n = 4x = 60 to 2n = 6x = 90, with a basic chromosome number of x = 15. The hypothetic allopolyploid origin and parental genomes of these polyploid types remains unknown. Genomic in situ hybridization (GISH) was used to study the genome organization and evolution of C. reticulata. Total genomic DNA from closely-related diploid species (C pitardii and C saluenensis), with the chromosome number 2n = 2x = 30, were labeled and hybridized in the presence of blocking DNA onto metaphase spreads of C reticulata. The C pitartii probe painted part of the tetraploid and hexaploid C reticulata genomes, whereas the C saluenensis probe delineated part of the hexaploid C reticulata genome. The results provide compelling evidence for the allopolyploid origin of C. reticultata genomes and demonstrate that 1) the diploid C. reticultata, C. pitardii and C. saluenensis are the progenitors of polyploid C reticultata, 2) hybridization between diploid C reticultata and diploid C pitardii gave birth to allotetraploid C. reticulata, and 3) subsequent hybridization between allotetraploid C. reticulata and diploid C. saluenensis formed the allohexaploid C. reticulata. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
关键词	Camellia Reticulata C. Pitardii C. Saluenensis Genomic In Situ Hybridization (Gish) Genome Donors
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.plantsci.2010.12.006
收录类别	SCI
语种	英语
WOS记录号	WOS:000287114500019
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/5307
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Li-Qin,Gu, Zhi-Jian. Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)[J]. PLANT SCIENCE,2011,180(3):554-559.
APA	Liu, Li-Qin,&Gu, Zhi-Jian.(2011).Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae).PLANT SCIENCE,180(3),554-559.
MLA	Liu, Li-Qin,et al."Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)".PLANT SCIENCE 180.3(2011):554-559.

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