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Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)
Liu, Li-Qin1,2; Gu, Zhi-Jian1
Corresponding AuthorLiu, LQ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2011-03-01
Source PublicationPLANT SCIENCE
ISSN0168-9452
Volume180Issue:3Pages:554-559
AbstractCamellia reticulata (Theaceae genus Camellia) is a world-famous, ornamental flowering plant. More interestingly, it has a polyploid series varying from 2n = 2x = 30, 2n = 4x = 60 to 2n = 6x = 90, with a basic chromosome number of x = 15. The hypothetic allopolyploid origin and parental genomes of these polyploid types remains unknown. Genomic in situ hybridization (GISH) was used to study the genome organization and evolution of C. reticulata. Total genomic DNA from closely-related diploid species (C pitardii and C saluenensis), with the chromosome number 2n = 2x = 30, were labeled and hybridized in the presence of blocking DNA onto metaphase spreads of C reticulata. The C pitartii probe painted part of the tetraploid and hexaploid C reticulata genomes, whereas the C saluenensis probe delineated part of the hexaploid C reticulata genome. The results provide compelling evidence for the allopolyploid origin of C. reticultata genomes and demonstrate that 1) the diploid C. reticultata, C. pitardii and C. saluenensis are the progenitors of polyploid C reticultata, 2) hybridization between diploid C reticultata and diploid C pitardii gave birth to allotetraploid C. reticulata, and 3) subsequent hybridization between allotetraploid C. reticulata and diploid C. saluenensis formed the allohexaploid C. reticulata. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
KeywordCamellia Reticulata C. Pitardii C. Saluenensis Genomic In Situ Hybridization (Gish) Genome Donors
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
DOI10.1016/j.plantsci.2010.12.006
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000287114500019
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/5307
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Li-Qin,Gu, Zhi-Jian. Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)[J]. PLANT SCIENCE,2011,180(3):554-559.
APA Liu, Li-Qin,&Gu, Zhi-Jian.(2011).Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae).PLANT SCIENCE,180(3),554-559.
MLA Liu, Li-Qin,et al."Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)".PLANT SCIENCE 180.3(2011):554-559.
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