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题名: Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique
作者: Sui, Xue-yi2; Huang, Yuan; Tan, Ying3; Guo, Yu2; Long, Chun-lin1, 4
刊名: PLANTA MEDICA
关键词: Sabia parviflora ; Sabiaceae ; DNA barcoding ; ethnomedicinal plant ; authentication
英文摘要: Sabia parviflora Wall. ex Roxb. is a traditional herb widely used by Chinese people, especially by the Buyi ethnic group which resides in Guizhou and Yunnan provinces. According to the Chinese Ethnic Pharmacopeia, the species is commonly used for soothing the liver and for the treatment of icteric hepatitis, hemostasis, and inflammation. However, due to the similar morphological characters of Sabia species and higher market demands, there are many substitutes and adulterants of S. parviflora. In this study, the differential identification of 6 Sabia species and 7 adulterants were investigated through DNA sequence analysis of three candidate DNA barcodes (trnH-psbA, rbcL-a, matK). Based on sequence alignments, we concluded that not only the trnH-psbA spacer sequence can distinguish S. parviflora from other Sabia species, but the matK + rbcL-a sequences also can differentiate it from the substitutes and adulterants. The classification tree of all samples based on rbcL-a sequences indicated that the rbcL region can identify samples into a family/genus level. Our results suggest that the three candidate barcodes can be used for the identification of S. parviflora and to distinguish it from common substitutes or adulterants.
出版日期: 2011-03-01
卷号: 77, 期号:5, 页码:492-496
语种: 英语
收录类别: SCI
学科分类: Plant Sciences; Pharmacology & Pharmacy
ISSN号: 0032-0943
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/5281
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650204, Yunnan, Peoples R China
2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
4.Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China

Recommended Citation:
Sui, XY; Huang, YA; Tan, Y; Guo, Y; Long, CL.Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique,PLANTA MEDICA,2011,77(5):492-496
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