Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique
Sui, Xue-yi2; Huang, Yuan; Tan, Ying3; Guo, Yu2; Long, Chun-lin1,4
通讯作者Long, CL (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, 132 Lanhei Rd, Kunming 650204, Yunnan, Peoples R China
2011-03-01
发表期刊PLANTA MEDICA
ISSN0032-0943
卷号77期号:5页码:492-496
摘要Sabia parviflora Wall. ex Roxb. is a traditional herb widely used by Chinese people, especially by the Buyi ethnic group which resides in Guizhou and Yunnan provinces. According to the Chinese Ethnic Pharmacopeia, the species is commonly used for soothing the liver and for the treatment of icteric hepatitis, hemostasis, and inflammation. However, due to the similar morphological characters of Sabia species and higher market demands, there are many substitutes and adulterants of S. parviflora. In this study, the differential identification of 6 Sabia species and 7 adulterants were investigated through DNA sequence analysis of three candidate DNA barcodes (trnH-psbA, rbcL-a, matK). Based on sequence alignments, we concluded that not only the trnH-psbA spacer sequence can distinguish S. parviflora from other Sabia species, but the matK + rbcL-a sequences also can differentiate it from the substitutes and adulterants. The classification tree of all samples based on rbcL-a sequences indicated that the rbcL region can identify samples into a family/genus level. Our results suggest that the three candidate barcodes can be used for the identification of S. parviflora and to distinguish it from common substitutes or adulterants.
关键词Sabia Parviflora Sabiaceae Dna Barcoding Ethnomedicinal Plant Authentication
学科领域Plant Sciences ; Pharmacology & Pharmacy
DOI10.1055/s-0030-1250468
收录类别SCI
语种英语
WOS记录号WOS:000288847600015
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/5281
专题资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650204, Yunnan, Peoples R China
2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
4.Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China
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GB/T 7714
Sui, Xue-yi,Huang, Yuan,Tan, Ying,et al. Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique[J]. PLANTA MEDICA,2011,77(5):492-496.
APA Sui, Xue-yi,Huang, Yuan,Tan, Ying,Guo, Yu,&Long, Chun-lin.(2011).Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique.PLANTA MEDICA,77(5),492-496.
MLA Sui, Xue-yi,et al."Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique".PLANTA MEDICA 77.5(2011):492-496.
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